Proteomic Study Identifies Proteins Involved in Brassinosteroid Regulation of Rice Growth

查看全文 作　　者：Fengru [1,2,6]Wang;Ming-Yi [3]Bai;Zhiping [3]Deng;Juan A. Oses-[4]Prieto;Alma L. [4]Burlingame;Tiegang [5]Lu;Kang [1]Chong;Zhi-Yong [3]Wang

机构地区：[1]Key Laboatory of PhotoSynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China;[2]Agriculture University of Hebei, Baoding 071001, China;[3]Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA;[4]Mass Spectrometry Facilityl Department of Pharmaceutical Chemistry, University of California, San Francisco CA 94143, USA;[5]Biotechnology Research Institute, the Chinese Academy of Agriculture Sciences, Beijing 100081, China;[6]Graduate University of the Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Journal of Integrative Plant Biology》索引2010年第52卷第12期,共11页

基　　金：supported by grants from US Department of Energy (DE-FG02-09ER15973);National Science Foundation of China (30870207);The UCSF Mass Spectrometry Facility (A.L. Burlingame, Director) was supported by the Biomedical Research Technology Program of the National Center for Research Resources, NIH NCRR P41RR001614, NIH NCRRRR015804, NIH NCRR P41RR019934 and RR012961 摘　　要：Brassinosteroids (BRs) are essential hormones for growth and development of plant. In rice, BRs regulate multiple developmental processes and affect many important traits such as height, leaf angle, fertility and seed filling. We identified brassinosteroid-regulated proteins in rice using proteomic approaches and performed functional analysis of some BR-regulated proteins by overexpression experiments. Using two-dimensional difference gel electrophoresis (2-D DIGE) followed by protein identification by mass spectrometry, we compared proteomic differences in the shoots and roots of the BR-insensitive mutant d61-4 and BR-deficient mutant brd1-3. We identified a large number of proteins differentially expressed in the mutants compared with wild type control. These include a glycine-rich RNA-binding protein (OsGRP1) and a DREPP2 protein, which showed reduced levels in the BR mutants. Overexpression of these two proteins partially suppressed the dwarf phenotype of the Arabidopsis BR-insensitive mutant bri1-5. In contrast to the reduced protein level, the RNA level of OsGRP1 was not significantly affected in the BR mutants or by BR treatment, suggesting BR regulation of OsGRP1 at the posttranslational level. This study identifies many BR-regulated proteins and demonstrates that OsGRP1 functions downstream in the BR signal transduction pathway to promote cell expansion. 关键词：蛋白质组学油菜素内酯水稻生长调控 RNA结合蛋白缺失突变体生物圈保护区信号转导通路

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Proteomic Study Identifies Proteins Involved in Brassinosteroid Regulation of Rice Growth

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