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Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of bacteria cultured in liquid media

查看全文 作　　者：ZHOU [1]Na;WANG [2]Na;XU [2]Bin;WANG [2]Jie;FANG [2]JunJian;DONG [2]FangTing;HE [2]Kun;YANG [1]XiaoHong 高影响力作者 机构地区：[1]Pharmaceutical Department of Jilin University, Changchun 130021, China;[2]National Center of Biomedical Analysis, Beijing 100850, China高影响力机构 出　　处：《Science China(Life Sciences)》索引2011年第54卷第1期,共6页高影响力期刊 基　　金：supported by the National Science and Technology Major Project (Grant No. 2008ZX10207);the Innovation Method Fund of Ministry of Science and Technology of China (Grant Nos. 2008IM022000 and 2009IM030300);the National Science and Technology Support Program (Grant No. 2008BAK41B0) 摘　　要：Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used for many years to rapidly identify whole bacteria. However, no consistent methodology exists for the rapid identification of bacteria cultured in liquid media. Thus, in this study we explored the use of MALDI-TOF MS analysis for rapid identification of cells cultured in liquid media. We determined that 2,5-dihydroxybenzoic acid (50 mg mL-1, 50% acetonitrile, 0.1% trifluoroacetic acid) was the best matrix solution for MALDI-TOF MS for this type of study. Moreover, the tested strains were successfully differentiated by principal component analysis, and the main characteristics of the mass peaks for each species were found in mixed culture samples. In addition, we found that the minimum number of cells for detection was 1.8×103. In conclusion, our findings suggest that MS-based techniques can be developed as an auxiliary method for rapidly and accurately identifying bacteria cultured in liquid media. 关 键 词：基质辅助激光解吸 细菌培养 飞行质谱 快速鉴定 全细胞 液体介质 时间 电离
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