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Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection

查看全文 作　　者：Qiu-lei [1,2]LANG;Xiao-chuan [3]ZHOU;Xiao-lin [3]ZHANG;Rafal [4]DRABEK;Zhi-xiang [3]ZUO;Yong-liang [3]REN;Tong-bin [3]LI;Ji-shuang [1,2]CHEN;Xiao-lian [3,4]GAO 高影响力作者 机构地区：[1]College of Life Sciences, Zhejiang University, Hangzhou 310058, China;[2]Institute of Bioengineering, Zhejiang Sci- Tech University, Hangzhou 310018, China;[3]LC Sciences, 2575 W. Bellfort, Suite 270, Houston, ZY 77054, USA;[4]Department of Biology and Biochemistry, University of Houston, Houston, TX 77004, USA高影响力机构 出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》索引2011年第12卷第2期,共10页高影响力期刊 基　　金：supported by the National High-Tech Research and Development Program (863) of China (No. 2008AA10Z129);the National Natural Science Foundation of China (No. 30800716) 摘　　要：A large number of plant microRNAs (miRNAs) are now documented in the miRBase, among which only 30 are for Solanum lycopersicum (tomato). Clearly, there is a far-reaching need to identify and profile the expression of miRNAs in this important crop under various physiological and pathological conditions. In this study, we used an in situ synthesized custom microarray of plant miRNAs to examine the expression and temporal presence of miRNAs in the leaves of tomato plants infected with Cucumber mosaic virus (CMV). Following computational sequence homology search and hairpin structure prediction, we identified three novel tomato miRNA precursor genes. Our results also show that, in accordance with the phenotype of the developing leaves, the tomato miRNAs are differentially expressed at different stages of plant development and that CMV infection can induce or suppress the expression of miRNAs as well as up-regulate some star miRNAs (miRNA*s) which are normally present at much lower levels. The results indicate that developmental anomalies elicited by virus infection may be caused by more complex biological processes. 关 键 词：茄属 lycopersicum 植物 miRNA 黄瓜马赛克病毒 miRNA 数组 miRNA 反应
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