Dynamic expression of P-glycoprotein in the CA1,CA3 and dentate gyrus of the rat hippocampus following lithium-pilocarpine-induced status epilepticus

查看全文 作　　者：Shuda Chen Jueqian Zhou Xiuling Liang Ziyi Chen Libai Yang Liemin [1]Zhou

机构地区：[1]Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China

出　　处：《Neural Regeneration Research》索引2010年第5卷第24期,共7页

基　　金：the Science and Technology Foundation of Guangdong Province,No.2009B060700049,2008B060600063;the National Natural Science Foundation of China,No.10671213 摘　　要：Epileptic seizures induce overexpression of P-glycoprotein in the blood-brain barrier. However,it is unclear whether hippocampal neurons also overexpress P-glycoprotein following seizure. This study confirmed that the clinical manifestation,pathological characteristics and electroencephalography in the rat model of lithium-pilocarpine-induced mesial temporal lobe epilepsy were consistent with clinical reports of mesial temporal lobe epilepsy in humans. Immunohistochemistry staining demonstrated that P-glycoprotein positive staining was found in neurons in the pyramidal layer of the hippocampus. Western blot assay and real-time polymerase chain reaction revealed that P-glycoprotein overexpression was exhibited in the CA1,CA3,and dentate gyrus of the hippocampus at 24 and 60 days following model induction,but no significant difference was detected in the same region at various time points. These results indicate that seizures led to overexpression of P-glycoprotein in neurons of the hippocampus,but no evidence was found for a positive association between P-glycoprotein expression and seizure frequency. 关键词：海马CA1区大鼠模型糖蛋白 CA3 癫痫诱发免疫组织化学染色动态表

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Dynamic expression of P-glycoprotein in the CA1,CA3 and dentate gyrus of the rat hippocampus following lithium-pilocarpine-induced status epilepticus

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