Chemometrics of differentially expressed proteins from colorectal cancer patients

查看全文 作　　者：Lay-Chin [1]Yeoh;Saravanan [2]Dharmaraj;Boon-Hui [3]Gooi;Manjit [3]Singh;Lay-Harn [1]Gam

机构地区：[1]School of Pharmaceutical Sciences,Universiti Sains Malaysia;[2]Centre for Drug Research,Universiti Sains Malaysia;[3]Department of Surgery,Penang General Hospital

出　　处：《World Journal of Gastroenterology》索引2011年第17卷第16期,共8页

基　　金：Supported by Research Universiti Grant,Grant No. 1001/PFAR MASI/815007 摘　　要：AIM:To evaluate the usefulness of differentially expressed proteins from colorectal cancer (CRC) tissues for differentiating cancer and normal tissues.METHODS:A Proteomic approach was used to identify the differentially expressed proteins between CRC and normal tissues.The proteins were extracted using Tris buffer and thiourea lysis buffer (TLB) for extraction of aqueous soluble and membrane-associated proteins,respectively.Chemometrics,namely principal component analysis (PCA) and linear discriminant analysis (LDA),were used to assess the usefulness of these proteins for identifying the cancerous state of tissues.RESULTS:Differentially expressed proteins identified were 37 aqueous soluble proteins in Tris extracts and 24 membrane-associated proteins in TLB extracts.Based on the protein spots intensity on 2D-gel images,PCA by applying an eigenvalue > 1 was successfully used to reduce the number of principal components (PCs) into 12 and seven PCs for Tris and TLB extracts,respectively,and subsequently six PCs,respectively from both the extracts were used for LDA.The LDA classification for Tris extract showed 82.7% of original samples were correctly classified,whereas 82.7% were correctly classified for the cross-validated samples.The LDA for TLB extract showed that 78.8% of original samples and 71.2% of the cross-validated samples were correctly classified.CONCLUSION:The classification of CRC tissues by PCA and LDA provided a promising distinction between normal and cancer types.These methods can possibly be used for identification of potential biomarkers among the differentially expressed proteins identified. 关键词：化学计量学方法蛋白质组学差异表达大肠癌组织状态可溶性蛋白患者线性判别分析

相关文献

参考文献（28）
引证文献（1）
耦合文献（2）

参考文献(28)

引证文献(1)

耦合文献(2)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Chemometrics of differentially expressed proteins from colorectal cancer patients

参考文献(28)

引证文献(1)

耦合文献(2)

用户提示