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Suppression of glutamate synthase genes significantly affects carbon and nitrogen metabolism in rice (Oryza sativa L.)

查看全文 作　　者：LU YongEn LUO Feng YANG Meng LI XiangHua LIAN [1]XingMing 高影响力作者 机构地区：[1]National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China高影响力机构 出　　处：《Science China(Life Sciences)》索引2011年第54卷第7期,共13页高影响力期刊 基　　金：supported by the National High Technology Research and Development Program of China (Grant No. 2010AA101802);Transgenic Project (Grant No. 2008ZX08001-005) 摘　　要：Rice (Oryza sativa) glutamate synthase (GOGAT,EC 1.4.1.14) enzymes have been proposed to have great potential for improving nitrogen use efficiency,but their functions in vivo and their effects on carbon and nitrogen metabolism have not been systematically explored.In this research,we analyzed transcriptional profiles of rice GOGAT genes using a genome-wide microarray database,and investigated the effects of suppression of glutamate synthase genes on carbon and nitrogen metabolism using GOGAT co-suppressed rice plants.Transcriptional profiles showed that rice GOGAT genes were expressed differently in various tissues and organs,which suggested that they have different roles in vivo.Compared with the wild-type,tiller number,total shoot dry weight,and yield of GOGAT co-suppressed plants were significantly decreased.Physiological and biochemical studies showed that the contents of nitrate,several kinds of free amino acids,chlorophyll,sugars,sugar phosphates,and pyridine nucleotides were significantly decreased in leaves of GOGAT co-suppressed plants,but the contents of free ammonium,2-oxoglutarate,and isocitrate in leaves were increased.We conclude that GOGATs play essential roles in carbon and nitrogen metabolism,and that they are indispensable for efficient nitrogen assimilation in rice. 关 键 词：谷氨酸合酶基因 氮肥利用效率 水稻 代谢影响 碳 植物叶片 ORYZA 硝酸盐含量
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