Screening of a Composite Microbial System and Its Characteristics of Wheat Straw Degradation

查看全文 作　　者：LI Pei-pei WANG Xiao-juan YUAN Xu-feng WANG Xiao-fen CAO Yan-zuan CUI Zong-[1]jun

机构地区：[1]College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China

出　　处：《Agricultural Sciences in China》索引2011年第10卷第10期,共9页

基　　金：supported by the National Key Technology R&D Program of China (2008BADC4B01 and 2008BADC4B17);the National Special Research Fund for Non-Profit Sector, China (200803033) 摘　　要：To accelerate the decomposition of wheat straw directly returned to soil, we constructed a microbial system (ADS-3) from agricultural soil containing rotting straw residues using a 40-wk limited cultivation. To assess its potential use for accelerating straw decomposing, the decomposing characteristics and the microbial composition of ADS-3 were analyzed. The results indicated that it could degrade wheat straw and filter paper by 63.8 and 80%, respectively, during 15 d of incubation. Straw hemicellulose degraded dramatically 51.2% during the first 3 d, decreasing up to 73.7% by the end of incubation. Cellulose showed sustained degradation reaching 53.3% in 15 d. Peak values of xylanase and cellulase activities appeared at 3 and 11 d, with 1.32 and 0.15 U mL-1, respectively. Estimated pH averaged 6.4-7.6 during the degradation process, which approximated acidity and alkalinity of normal soils. The microbial composition of ADS-3 was stable based on denaturing gradient gel electrophoresis (DGGE) analysis. By using bacterial 16S rRNA and fungal 26S rRNA gene clone library analysis, 20 bacterial clones and 7 fungal clones were detected. Closest identified relatives of bacteria represented by Bacillus fusiformis, Cytophaga sp., uncultured Clostridiales bacterium, Ruminobacillus xylanolyticum, Clostridium hydroxybenzoicum, and uncultured proteobacterium and the fungi were mainly identified as related to Pichia sp. and uncultured fungus. 关键词：微生物系统退化变性梯度凝胶电泳 RRNA基因微生物组成小麦秸秆麦草筛选

相关文献

参考文献（32）
引证文献（17）
耦合文献（298）

参考文献(32)

引证文献(17)

耦合文献(298)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Screening of a Composite Microbial System and Its Characteristics of Wheat Straw Degradation

参考文献(32)

引证文献(17)

耦合文献(298)

用户提示