寿光黑鸡成纤维细胞的体外培养与冷冻保存(英文)

查看全文 作　　者：[1]王娟;[2]于媛;[1]王跃嗣;[3]马云;[2]焦飞

机构地区：[1]滨州医学院药学院细胞工程教研室,山东烟台264003;[2]滨州医学院基础学院生物化学教研室,山东烟台264003;[3]滨州医学院基础学院病理教研室,山东烟台264003

出　　处：《Agricultural Science & Technology》索引2008年第9卷第6期,共6页

基　　金：Supported by National Natural Science Foundation of China(30801353);Shandong Education Department Foundation Project(G08LG53)~~ 摘　　要：[Objective] The aim of this study was to establish the in vitro culture system of chicken fibroblasts.[Method] Tissue explant method and enzymatic digestion method were used to separate and culture chicken skin fibroblasts respectively.The rate of cell growth,cryopreservation and recovery were compared.[Result] The primary chicken fibroblasts prepared by enzymatic digestion grew faster and converged together to form monolayer on 5 d post preparation;the passage cells prepared by these 2 methods grew at similar speed and formed monolayer within 2-3 d;homogeneous fibroblasts could be obtained by trypsin digestion and repeated attachment for 3-4 passages;there were 75%-80% of cells survived after cryopreservation and recovery;the growth curves of embryonic fibroblasts and skin fibroblasts were all normal and the two kind of cells still retained the normal number of chromosomes even at the twelfth passage.[Conclusion] The feeder layer cells needed for establishing ES cell lines could be obtained by culturing chicken fibroblasts through both tissue explant method and enzymatic digestion method.This study provided a basis for the successful establishment of ES cell lines. 关键词：SKIN EMBRYONIC FIBROBLASTS Culture in VITRO CRYOPRESERVATION

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寿光黑鸡成纤维细胞的体外培养与冷冻保存(英文)

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