Expression Patterns of Cytochrome P450 Aromatase Genes During Ovary Development and Their Responses to Temperature Stress in Female Yellow Catfish (Pelteobagrus fulvidraco)

查看全文 作　　者：LIU [1]Miao;WEN [1]Haishen;HE [1]Feng;LI [1]Jifang;SHI [1]Dan;HU [1]Jian;ZHANG [1]Yuanqing;MA [1]Ruiqin;MU [1]Weijie;QI [2]Baoxia

机构地区：[1]Fisheries College,Ocean University of China,Qingdao 266003,P.R.China;[2]College of Animal Science and Technology,Inner Mongolia Nationality University,Tongliao 028000,P.R.China

出　　处：《Journal of Ocean University of China》索引2011年第10卷第4期,共8页

基　　金：supported by the Key Open Fund of the Key Open Laboratory of Genetic Breeding;Aquaculture Biology of Aquatic Animal, Ministry of Agriculture, China (BZ200709) 摘　　要：Cytochrome P450 aromatase(P450arom)plays a pivotal role in ovary development.In this study,we used semi-quanti-tative reverse transcription PCR(RT-PCR)to analyze spatiotemporal expressions of two P450arom genes(CYP19A and CYP19B)and their responses to temperature stress in female yellow catfish(Pelteobagrus fulvidraco).Tissue distribution pattern of CYP19 showed that CYP19B was abundantly expressed in fish brain and ovary(brain > ovary),but weakly in intestines,whereas CYP19A was exclusively expressed in ovary.Semi-quantitative RT-PCR analyses showed high transcript abundance of both CYP19A and CYP19B in the ovarian reproductive cycle,corresponding with serum estradiol-17β(E2)levels.Increases in aromatases,serum E2 and testosterone(T)levels in fish exposed to higher temperature indicated stimulation of ovarian maturation and recrudescence by heat stress in stages Ⅱ and Ⅴ during the ovarian cycle,whereas associated decreases in stage Ⅲ suggested vitellogenesis inhibition by heat stress.Gene expression of CYP19 was closely related to levels of serum E2.Results demonstrated CYP19 played a crucial role in the reproductive cycle of female yellow catfish.Different temperature stress affected CYP19 gene expression in the fish ovarian re-productive cycle.Associated P450arom genes could be useful for studying physiological aspects of yellow catfish. 关键词：P450AROM ESTROGENS TESTOSTERONE OVARY temperature

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Expression Patterns of Cytochrome P450 Aromatase Genes During Ovary Development and Their Responses to Temperature Stress in Female Yellow Catfish (Pelteobagrus fulvidraco)

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