Determination of levels of adenosine phosphates in blood by ion chromatography

查看全文 作　　者：Pei Jin [1]Xie;Ming Li [1,2]Ye;Zhong Yang [2]Hu;Guang Wen [2]Pan;Yan [1]Zhu;Jia Jie [1]Zhang

机构地区：[1]Department of Chemistry,Zhejiang University,Hangzhou 310028,China;[2]Shanghai Lab,Dionex China Co.,Ltd.,Shanghai 201203,China

出　　处：《Chinese Chemical Letters》索引2011年第22卷第12期,共4页

基　　金：financially supported by National Natural Science Foundation of China(Nos.20775070 and J0830413);Zhejiang Provincial Natural Science Foundation of China(Nos.R4080124 and Y4090268) 摘　　要：A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18(50 mm×4 mm) guard column and IonPac AS 18(250 mm×4 mm) analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits(S/N=3) of AMP,ADP and ATP were 38,47,108 ng/L,respectively.The relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was obtained.This method was applied to analyze human blood samples. 关键词：ATP水平三磷酸腺苷血液样本同时测定离子色谱法阴离子交换色谱法 IonPac 电导检测器

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Determination of levels of adenosine phosphates in blood by ion chromatography

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