ZmcrtRB3 Encodes a Carotenoid Hydroxylase that Affects the Accumulation of α-carotene in Maize Kernel

查看全文 作　　者：Yi [1]Zhou;Yingjia [1]Han;Zhigang [1]Li;Yang [1]Fu;Zhiyuan [1,2]Fu;Shutu [1]Xu;Jiansheng [1]Li;Jianbing [1,3]Yan;Xiaohong [1]Yang

机构地区：[1]National Maize Improvement Center,Key Laboratory of Crop Genomics and Genetic Improvement,China Agricultural University,100193 Beijing,China;[2]Zhengzhou Sub-center of Chinese National Maize Improvement Center,Henan Agricultural University,Zhengzhou 450002,China;[3]National Key Laboratory of Crop Improvement,Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Journal of Integrative Plant Biology》索引2012年第54卷第4期,共10页

基　　金：supported by the National Hi-Tech Research and Development Program of China (863 Program,2012AA10A307);the National Natural Science Foundation of China(30821140352,31123009);the HarvestPlus Challenge Program in China 摘　　要：α-carotene is one of the important components of pro-vitamin A,which is able to be converted into vitamin A in the human body.One maize(Zea mays L.) ortholog of carotenoid hydroxylases in Arabidopsis thaliana,ZmcrtRB3,was cloned and its role in carotenoid hydrolyzations was addressed.ZmcrtRB3 was mapped in a quantitative trait locus(QTL) cluster for carotenoid-related traits on chromosome 2(bin 2.03) in a recombinant inbred line(RIL) population derived from By804 and B73.Candidate-gene association analysis identified 18 polymorphic sites in ZmcrtRB3 significantly associated with one or more carotenoid-related traits in 126 diverse yellow maize inbred lines.These results indicate that the enzyme ZmcrtRB3 plays a role in hydrolyzing both α-and β-carotenes,while polymorphisms in ZmcrtRB3 contributed more variation in α-carotene than that in β-carotene.Two single nucleotide polymorphisms(SNPs),SNP1343 in 5 untranslated region and SNP2172 in the second intron,consistently had effects on α-carotene content and composition with explained phenotypic variations ranging from 8.7% to 34.8%.There was 1.7-to 3.7-fold change between the inferior and superior haplotype for α-carotene content and composition.Thus,SNP1343 and SNP2172 are potential polymorphic sites to develop functional markers for applying marker-assisted selection in the improvement of pro-vitamin A carotenoids in maize kernels. 关键词：类胡萝卜素玉米籽粒羟化酶分子标记辅助选择 β-胡萝卜素多态性位点单核苷酸多态性积累

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ZmcrtRB3 Encodes a Carotenoid Hydroxylase that Affects the Accumulation of α-carotene in Maize Kernel

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