Epigenetic reprogramming of embryos derived from sperm frozen at-20℃

查看全文 作　　者：CHAO [1,2]ShiBin;LI [1]JianChun;JIN [1]XuanJin;TANG [1]HaiXun;WANG [1]GongXian;GAO [2]GuoLan

机构地区：[1]Center of Reproductive Medicine, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China;[2]Department of Obstetrics and Gynecology, Aviation General Hospital of China, Beijing 100101, China

出　　处：《Science China(Life Sciences)》索引2012年第55卷第4期,共9页

基　　金：supported by the National Basic Research Program of China (Grant No. 2011CB944501) 摘　　要：Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after application of intracytoplasmic sperm injection (ICSI) is much needed. It has been shown previously that spermatozoa frozen at 20°C can activate oocytes and support full-term embryo development. However, epigenetic reprogramming could be affected by the environment and by the in vitro manipulation of gametes. Here, we investigated embryo epigenetic reprogramming including DNA methylation and histone modification, in embryos derived from sperm preserved at 20°C without cryoprotectants. The results showed that although both fertilization and embryo developmental competence were decreased, the dynamic epigenetic reprogramming of embryos derived from frozen sperm was similar to the reprogramming of embryos derived from fresh sperm. The results reported in this study indicate that sperm frozen without cryoprotectant is epigenetically safe for ICSI. 关键词：精子保存胚胎冷冻遗传修饰表观冷冻保护剂 DNA甲基化冷冻保存动物种类

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Epigenetic reprogramming of embryos derived from sperm frozen at-20℃

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