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Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats

查看全文 作　　者：Fengguo [1]Zhai;Xiuping [1]Zhang;Yue [2]Guan;Xudong [1]Yang;Yang [3]Li;Gaochen [4]Song;Lixin [1]Guan 高影响力作者 机构地区：[1]Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China;[2]Department of Endocrinology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China;[3]Department of Thoracic Surgery, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China;[4]Department of Biochemistry, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, China高影响力机构 出　　处：《Neural Regeneration Research》索引2012年第7卷第12期,共7页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China,No.30973897;the Natural Science Foundation of Heilongjiang Province,No.D200978;the Postgraduate Innovative Scientific Research Foundation of Heilongjiang Province,No.YJSCX2011-286HLJ 摘　　要：Rat models of focal cerebral ischemia/reperfusion injury were established by occlusion of the middle cerebral artery.Microarray analysis showed that 24 hours after cerebral ischemia,there were nine up-regulated and 27 down-regulated microRNA genes in cortical tissue.Bioinformatic analysis showed that bcl-2 was the target gene of microRNA-384-5p and microRNA-494,and caspase-3 was the target gene of microRNA-129,microRNA-320 and microRNA-326.Real-time PCR and western blot analyses showed that 24 hours after cerebral ischemia,bcl-2 mRNA and protein levels in brain tissue were significantly decreased,while caspase-3 mRNA and protein levels were significantly increased.This suggests that following cerebral ischemia,differentially expressed microRNA-384-5p,microRNA-494,microRNA-320,microRNA-129 and microRNA-326 can regulate bcl-2 and caspase-3 expression in brain tissue. 关 键 词：缺血/再灌注损伤 小分子RNA CASPASE-3 大脑 表达谱 bcl-2 BLOT分析 mRNA
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