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Serum containing Tongqiaohuoxue decoction suppresses glutamate-induced PC12 cell injury

查看全文 作　　者：Ning [1,2,3]Wang;Yi [3]Dengt;Wei [1]Wei;Lihua [2]Song;Yan [3]Wang 高影响力作者 机构地区：[1]Institute of Clinical Pharmacology of Anhui Medical University, Hefei 230032, Anhui Province, China2Anhui Anke Biotechnoloov (Grouo) Co.. Ltd.. Hefei 230088. Anhui Province. China;[2]Anhui Anke Biotechnology (Group) Co., Ltd., Hefei 230088, Anhui Province, China;[3]Anhui Key Laboratory of Modernized Chinese Material, Anhui University of Traditional Chinese Medicine, Hefei 230031, Anhui Province,China高影响力机构 出　　处：《Neural Regeneration Research》索引2012年第7卷第15期,共7页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China, No. 30973979;the Science-Technology Foundation for Excellent Young Scholar of Anhui Province China, No. 10040606Y17;Kanion Innovation Fund of Traditional Chinese Medicine, No. KYCX2010007 摘　　要：Glutamate application is an established method of inducing PC12 cell injury. PC12 cells were cultured with serum containing Tongqiaohuoxue decoction consisting of moschus, Carthamus tinctorius, Rhizoma chuanxiong, Semen pruni persicae, and Radix Paeoniae Rubra. After 24 hours of co-cultivation, glutamate (12.5 mM) was added to the culture medium. We found that serum containing Tongqiaohuoxue decoction prevented the increase in reactive oxygen species, and the decreases in superoxide dismutase and Na+-K+-ATPase activity, induced by glutamate. It also reduced the concentration of malondialdehyde, enhanced the mitochondrial transmembrane potential, inhibited the elevation of cellular calcium, and decreased phosphorylation of calmodulin-dependent protein kinase II. Thus, serum containing Tongqiaohuoxue decoction had protective effects on cell proliferation and membrane permeability in glutamate-injured PC12 cells. 关 键 词：PC12细胞 细胞损伤 谷氨酸 活血 诱导 血清 超氧化物歧化酶 ATP酶活性
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