Screening and Insert Size Analysis of Candidate C-Genome BAC Clones from Brassica napus

查看全文 作　　者：YAN [1]Xiaohong;YUAN [1]Yabin;ZHAO [1]Xueya;ZHA [1]Minmin;DAI [1]Shuang;LIU [1]Feng;WANG [1]Yuantao;WEI [1]Wenhui

机构地区：[1]Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,Hubei,China

出　　处：《Wuhan University Journal of Natural Sciences》索引2012年第17卷第4期,共6页

基　　金：Supported by the National Natural Science Foundation of China (30671312);the Natural Science Foundation of Hubei Province(2008CDA083 and 2009CDB191);the Chenguang Program of Wuhan City(201050231022) 摘　　要：Thirty-two C-genome specific candidate bacterial artificial chromosome (BAC) clones were successfully screened from the BAC library by four-dimensional PCR method with the primer pairs of 75 simple sequence repeat (SSR) markers located in the nine C-genome linkage groups of Brassica napus. The screened 32 BAC clones have an average insert size of 114.2 kb with a range of 30-190 kb. They are the first set of C-genome BAC clones screened from B. napus genomic BAC library. The average insert size of this set of BAC clones presented that the constructed BAC library had a high quality. This set of BAC clones can be used as markers to identify individual chromosomes of B. napus C-genome. 关键词：Brassica napus C-genome simple sequence repeat (SSR) marker four-dimensional PCR bacterial artificial chromo- some (BAC) clone

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Screening and Insert Size Analysis of Candidate C-Genome BAC Clones from Brassica napus

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