Antigenic epitope peptides of influenza H3N2 virus neuraminidase gene based on experiments

查看全文 作　　者：ZHONG [1,2]Jing;HUANG [1,3]Ping;WEN [4]MiaoHeng;LIANG [1,3]LiJun;ZHANG [1]Xin;TAN [4]SongNuan;ZHU [1]XiaoLan

机构地区：[1]Key Laboratory for Emergency Pathogen Detection, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510300, China;[2]Department of Infectious Diseases, Guangzhou Chest Hospital, Guangzhou 510095, China;[3]School of Public Health, Sun Yat-sen University, Guangzhou 510080, China;[4]Sinoasis Pharmaceuticals (Guangzhou) Inc, Guangzhou 510760, China

出　　处：《Chinese Science Bulletin》索引2012年第57卷第22期,共6页

基　　金：supported by the National Natural Science Foundation of China(30972757) 摘　　要：The neuraminidase (NA) in viral surface is one of the main subtype-specific antigen of influenza type A viruses.Neuraminidase is an enzyme to break the bonds between hemagglutinin (HA) and sialic acid to release newly formed viruses from infected cells.In this study,the H3N2 subtype virus NA genes were sequenced and NA proteins were screened for B-cell epitopes and assessed based on immunoinformatics.Based on this results,four peptides DR6,EY7,VG8 and RE8 (covering amino acid residues 151-156,368-374,398-405 and 428-435,respectively) of the NA protein were synthesized artificially.These peptides were used to immunize New Zealand rabbits subcutaneously to raise antisera.Experimental results showed that these four peptides were capable of eliciting antibodies against H3N2 viruses in a specific and sensitive feature,detected in vitro by enzyme-linked immunosorbent assay.Moreover,hemadsorption anti-releasing effects took place in three three-antisera-mixtures at a dilution of 1:40.Alignment using NA gene database showed that amino acid residues in these four epitope peptides were substituted at specific sites in all the NAs sequenced in this study.It was suggested that these NA epitope peptides might be used in combination with HA proteins as vaccine antigens. 关键词：神经氨酸酶基因 A型流感病毒抗原表位胜肽实验 NA基因特异性抗原氨基酸残基

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Antigenic epitope peptides of influenza H3N2 virus neuraminidase gene based on experiments

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