DNA methylation in tissues of Chamaedorea elegans

查看全文 作　　者：LU [1]Yongquan;QING [1]Jia;LI [1]Haiying;TONG [1]Zaikang

机构地区：[1]The Nurturing Station for the State Key Laboratory of Subtropical Silviculture,Zhejiang Agriculture and Forestry University,Lin'an 311300,China.

出　　处：《Journal of Forestry Research》索引2012年第23卷第3期,共4页

基　　金：supported by the Natural Science Foundation of China (30800879) 摘　　要：DNA methylation plays a crucial role in regulating plant development and tissue differentiation. In this study, we compared them ethylation levels in leaf, root, and stem in Chamaedorea elegans byusing the technique of methylation-sensitive amplified fragment length polymorphism AFLP. Over 19% (42/220) bases were uniformly methylated in these tissues. The percentages of polymorphism resulting from varied methylation in mature leaf (L1), young leaf (L2), baby leaf (L3),stem (S), young root (R1) and lignified root (R2) were 29.5%, 29.0%,27.1%, 30.7%, 63.0% and 28.3%, respectively. The numbers of polymorphic loci detected in the leaves of three developmental stages were similar, ranging from 20 to 30. In contrast, roots at the two developmental stages differed greatly, with 145 polymorphic loci detected in R1 and 27 in R2. Our results suggest that the methylation level in leaves slightly increases with aging, while that in roots decreases dramatically with aging. 关键词：DNA甲基化组织分化扩增片段长度多态性植物生长发育 AFLP技术多态位点甲基化水平发育阶段

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DNA methylation in tissues of Chamaedorea elegans

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