Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis

查看全文 作　　者：Peng [1,3]Cui;Wanfei [1,4]Liu;Yuhui [1,4]Zhao;Qiang [1,4]Lin;Daoyong [2]Zhang;Feng [1,3]Ding;Chengqi [1]Xin;Zhang [3]Zhang;Shuhui [1]Song;Fanglin [2]Sun;Jun [1]Yu;Songnian [1]Hu

机构地区：[1]CAS Key Laboratory of Genome Sciences and Information,Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing 100029,China;[2]Institute of Epigenetics and Cancer Research,School of Medicine,Tsinghua University,Beijing 100080,China;[3]Computational Bioscience Research Center,4700 King Abdullah University of Science and Technology,Thuwa123955-6900,Kingdom of Saudi Arabia;[4]Graduate University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Genomics, Proteomics & Bioinformatics》索引2012年第10卷第2期,共12页

基　　金：supported by Grants from Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-EW-R-01-04);National Science and Technology Key Project (2008ZX1004-013);863 Program(2009AA01A130);Special Foundation Work Program(2009FY120100);National Key Technology R&D Program (2008BA164B02);973 Program (2011CB944100,2011CB965300 and 2007CB948101) from the Ministry of Science and Technology of the People’s Republic of China 摘　　要：The global features of H3K4 and H3K27 trimethylations(H3K4me3 and H3K27me3) have been well studied in recent years,but most of these studies were performed in mammalian cell lines.In this work,we generated the genome-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChIP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLiD technology.We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression,function and development.Moreover,we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes,which contradicts the findings in previous studies.Finally,we observed that bivalent domains,with both H3K4me3 and H3K27me3,existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes.However,the bivalent domains tend towards a 'winner-takes-all' approach to regulate the expression of associated genes.We also verified the above results in mouse ES cells.As expected,the results in ES cells are consistent with those in cerebrum and testis.In conclusion,we present two very important findings.One is that H3K4me3 and H3K27me3 rarely occur in silent genes.The other is that bivalent domains may adopt a 'winner-takes-all' principle to regulate gene expression. 关键词：睾丸大脑小鼠组织特异性表达发育相关基因二价染色体 ES细胞全局特征

相关文献

参考文献（43）
引证文献（1）
耦合文献（0）

参考文献(43)

引证文献(1)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis

参考文献(43)

引证文献(1)

用户提示