Cell sheet technology for regeneration of esophageal mucosa

查看全文 作　　者：Ryo [1]Takagi;Masayuki [1]Yamato;Nobuo [1,2]Kanai;Daisuke [1,3]Murakami;Makoto [1,3]Kondo;Takaaki [1,3]Ishii;Takeshi [1,2]Ohki;Hideo [3]Namiki;Masakazu [2]Yamamoto;Teruo [1]Okano

机构地区：[1]Institute of Advanced Biomedical Engineering and Science,Tokyo Women's Medical University;[2]Department of Surgery,Institute of Gastroenterology,Tokyo Women's Medical University;[3]Graduate School of Science and Engineering,Waseda University

出　　处：《World Journal of Gastroenterology》索引2012年第18卷第37期,共6页

基　　金：Supported by The Formation of Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science;Technology from the Ministry of Education, Culture, Sports, Science, and Technology, Japan 摘　　要：The progress of tissue-engineering technology has realized development of new therapies to treat various disorders by using cultured cells. Cell-and tissue-based therapies have been successfully applied to human patients, and several tissue-engineered products have been approved by the regulatory agencies and are commercially available. In the review article, we describe our experience of development and clinical application of cell sheet-based regenerative medicine.Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) have been shown to be useful for removal of gastrointestinal neoplasms with less invasiveness compared with open surgery, especially in esophageal surgery. However, postoperative inflammation and stenosis are major complications observed after intensive mucosal resection. Therefore, we have developed novel regenerative medicine to prevent such complications and promote wound healing of esophageal mucosa after EMR or ESD. Transplantable oral mucosal epithelial cell sheets were fabricated from patients' own oral mucosa. Immediately after EMR or ESD, fabricated autologous cell sheets were endoscopically transplanted to the ulcer sites. We performed a preclinical study with a canine model. In human clinical settings, cell culture and cell sheet fabrication were performed in clean rooms according to good manufacturing practice guidelines, and pharmaceutical drugs were used as supplements to culture medium in place of research regents used in animal study. We believe that cell-based regenerative medicine would be useful to improve quality of life of patients after EMR or ESD. 关键词：口腔黏膜食管癌再生技术电池板上皮细胞临床应用再生医学胃肠道肿瘤

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Cell sheet technology for regeneration of esophageal mucosa

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