Detection of Penicillin via Surface Plasmon Resonance Biosensor

查看全文 作　　者：ZHANG [1]Ying;JIN [1]Wei;YANG Meng-[1]chao;ZHANG Ti-[1]qiang;ZHOU [1]Chao;XIE [1]Fei;SONG [1]Qi;REN [1]Hao;JIN Qin-[1]han;MU [1]Ying

机构地区：[1]Research Center for Analytical Instrumentation,Institute of Cyber-systems and Control,State Key Laboratory of Industrial Control Technology,Department of Control Science and Technology,Zhejiang University,Hangzhou 310058,P,R.China

出　　处：《Chemical Research in Chinese Universities》索引2012年第28卷第5期,共5页

基　　金：Supported by the National Natural Science Foundation of China(No.31070772);the Science and Technology Programs of Zhejiang Province, China(No.2011C37029);the Doctoral Program of Higher Education of China(No.200901011110136);the Science and Technology Programs of Suzhou City, China(No.ZXG0920) 摘　　要：A method of using Au colloid to capture the decomposed product of penicillin, penicillamine, on a surface plasmon resonance(SPR) biosensor for the quantitative determination of penicillin was developed. Based on the de-composition of penicillin to generate penicillamine and penilloaldehyde, a high sensitive biosensor for detecting penicillin was also developed. In our experiment, it was penicillamine rather than penicillin that has been measured. This is because penicillamine contains a functional group that makes it self-assembling on Au colloid to increase the molecular weight so as to improve the surface plasmon resonance signal. On a UV-Vis spectrophotometer, a high concentration of penicilliamine-Au complex was determined, indicating that penicillamine was already well combined with Au colloid. The method, using the combination of Au colloid with penicillamine, proved to detect penicillin. 关键词：表面等离子体共振传感器检测生物传感器青霉素 UV-VIS 定量测定青霉胺分光光度计

相关文献

参考文献（29）
引证文献（2）
耦合文献（0）

参考文献(29)

引证文献(2)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Detection of Penicillin via Surface Plasmon Resonance Biosensor

参考文献(29)

引证文献(2)

用户提示