Schizandra arisanensis extract attenuates cytokine-mediated cytotoxicity in insulin-secreting cells

查看全文 作　　者：Yi-Shin [1]Hsu;Yao-Haur [1,2]Kuo;Hui-Ling [2]Cheng;Peter R [3]Flatt;Hui-Kang [1]Liu

机构地区：[1]Division of Herbal Drugs and Natural Products, National Research Institute of Chinese Medicine;[2]Graduate Institute of Integrated Medicine, China Medical University;[3]School of Biomedical Sciences, University of Ulster

出　　处：《World Journal of Gastroenterology》索引2012年第18卷第46期,共10页

基　　金：Supported by National Science Council, No. NSC94-2314-B-077-001, No. NSC101-2320-B-077-003-MY2;National Research Institute of Chinese Medicine, No. NRICM95-DHM-04 摘　　要：AIM: To explore the bioactivity of an ethanolic extract of Schizandra arisanensis (SA-Et) and isolated constituents against interleukin-1 β and interferon-γ-mediated β cell death and abolition of insulin secretion. METHODS: By employing BRIN-BD11 cells, the effects of SA-Et administration on cytokine-mediated cell death and abolition of insulin secretion were evaluated by a viability assay, cell cycle analysis, and insulin assay. The associated gene and protein expressions were also measured. In addition, the bioactivities of several peak compounds collected from the SA-Et were tested against cytokine-mediated β cell death.RESULTS: Our Results revealed that SA-Et dose-dependently ameliorated cytokine-mediated β cell death and apoptosis. Instead of suppressing inducible nitric oxide synthase/nitric oxide cascade or p38MAPK activity, suppression of stress-activated protein kinase/c-Jun NH2-terminal kinase activity appeared to be the target for SA-Et against the cytokine mix. In addition, SA-Et provided some insulinotropic effects which re-activated the abolished insulin exocytosis in cytokine-treated BRIN-BD11 cells. Finally, schiarisanrin A and B isolated from the SA-Et showed a dose-dependent protective effect against cytokine-mediated β cell death. CONCLUSION: This is the first report on SA-Et ameliorating cytokine-mediated β cell death and dysfunction via anti-apoptotic and insulinotropic actions. 关键词：胰岛素分泌细胞毒作用细胞因子乙醇提取物北五味子阿里山介导诱导型一氧化氮合酶

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Schizandra arisanensis extract attenuates cytokine-mediated cytotoxicity in insulin-secreting cells

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