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Adenosine triphosphate promotes locomotor recovery after spinal cord injury by activating mammalian target of rapamycin pathway in rats

查看全文 作　　者：Zhengang [1,2]Sun;Lingyun [2,3]Hu;Yimin [2,4]Wen;Keming [4]Chen;Zhenjuan [5]Sun;Haiyuan [2]Yue;Chao [2]Zhang 高影响力作者 机构地区：[1]Department of Spine Surgery, First People's Hospital of Qingdao Economic & Technological Development Area;[2]Second Clinical Medical College, Lanzhou University;[3]Department of Orthopedics, Nanchong Central Hospital;[4]Department of Spine Surgery, Lanzhou General Hospital of Lanzhou Military Region;[5]Department of Information, Qingdao Eighth People's Hospital高影响力机构 出　　处：《Neural Regeneration Research》索引2013年第8卷第2期,共10页高影响力期刊 摘　　要：The mammalian target of rapamycin (mTOR) pathway plays an important role in neuronal growth, proliferation and differentiation. To better understand the role of mTOR pathway involved in the induction of spinal cord injury, rat models of spinal cord injury were established by modified Allen's stall method and interfered for 7 days by intraperitoneal administration of mTOR activator adenosin triphosphate and mTOR kinase inhibitor rapamycin. At 1-4 weeks after spinal cord injury induction, the Basso, Beattie and Bresnahan locomotor rating scale was used to evaluate rat locomotor function, and immunohistochemical staining and western blot analysis were used to detect the expression of nestin (neural stem cell marker), neuronal nuclei (neuronal marker), neuron specific enolase, neurofilament protein 200 (axonal marker), glial fibrillary acidic protein (astrocyte marker), Akt, mTOR and signal transduction and activator of transcription 3 (STAT3). Results showed that adenosine triphosphate-mediated Akt/mTOR/STAT3 pathway increased endogenous neural stem cells, induced neurogenesis and axonal growth, inhibited excessive astrogliosis and improved the locomotor function of rats with spinal cord injury. 关 键 词：转录激活因子 三磷酸腺苷 脊髓损伤 运动功能 大鼠模型 雷帕霉素 哺乳动物 神经元特异性烯醇化酶
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