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Arabidopsis RING Peroxins are E3 Ubiquitin Ligases that Interact with Two Homologous Ubiquitin Receptor Proteins

查看全文 作　　者：Navneet [1]Kaur;Qingzhen [2]Zhao;Qi [2]Xie;Jianping [1]Hu 高影响力作者 机构地区：[1]MSU-DOE Plant Research Laboratory, Michigan State University;[2]Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences高影响力机构 出　　处：《Journal of Integrative Plant Biology》索引2013年第55卷第1期,共13页高影响力期刊 基　　金：supported by grants from the National Science Foundation Arabidopsis 2010 program (MCB 0618335);the Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, Office of Science, US Department of Energy (DE-FG02–91ER20021) to J. H 摘　　要：Peroxisomes are essential eukaryotic organelles that mediate various metabolic processes. Peroxisome import depends on a group of peroxisome biogenesis factors called peroxins, many of which are evolutionarily conserved. PEX2, PEX10, and PEX12 are three RING-finger-domain-containing integral membrane peroxins crucial for protein import. In yeast (Saccharomyces cerevisae), RING peroxins act as E3 ligases, facilitating the recycling of the peroxisome import receptor protein PEX5 through ubiquitination. In plants, RING peroxins are essential to plant vitality. To elucidate the mode of action of the plant RING peroxins, we employed in vitro assays to show that the Arabidopsis RING peroxins also have E3 ligase activities. We also identified a PEX2-interacting protein, DSK2b, which is a member of the ubiquitin receptor family known to function as shuttle factors ferrying polyubiquitinated substrates to the proteasome for degradation. DSK2b and its tandem duplicate DSK2a are localized in the cytosol and the nucleus, and both interact with the RING domain of PEX2 and PEX12. DSK2 artificial microRNA lines did not display obvious defects in plant growth or peroxisomal processes, indicating functional redundancies among Arabidopsis ubiquitin receptor proteins. Our results suggest that Arabidopsis RING peroxins can function as E3 ligases and act together with the ubiquitin receptor protein DSK2 in the peroxisomal membrane-associated protein degradation system. 关 键 词：泛素连接酶 受体蛋白 拟南芥 过氧化物酶体 microRNA 植物生长 同源 交互
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