Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45

查看全文 作　　者：Hai-hong [1,2]ZHANG;Ai-zhen [2]CA;Xue-ming [1]WEI;Li [1]DING;Feng-zhi [1]LI;Ai-ming [1]ZHENG;Da-jiang [1]DAI;Rong-rong [1]HUANG;Hou-jun [1]CAO;Hai-yang [1]ZHOU;Jian-mei [1]WANG;Xue-jing [1]WANG;Wei [1]SHI;Heng [3]ZHU;Xiao-ying [1]YUAN;Lin [2]CHEN

机构地区：[1]Department of General Surgery,General Hospital of the Air Force,PLA;[2]Department of General Surgery,Chinese PLA General Hospital;[3]Department of Cell Biology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》索引2013年第14卷第3期,共8页

基　　金：Project (Nos.81000706/H1108 and 81172368) supported by the National Natural Science Foundation of China 摘　　要：Objective:Side population(SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer.Many kinds of cell lines and tissues have demonstrated the presence of SP cells,including several gastric cancer cell lines.This study is aimed to identify the cancer stem-like cells in the SP of gastric cancer cell line MKN-45.Methods:We used fluorescence activated cell sorting(FACS) to sort SP cells in the human gastric carcinoma cell line MKN-45(cells labeled with Hoechst 33342) and then characterized the cancer stem-like properties of SP cells.Results:This study found that the SP cells had higher clone formation efficiency than major population(MP) cells.Five stemness-related gene expression profiles,including OCT-4,SOX-2,NANOG,CD44,and adenosine triphosphate(ATP)-binding cassette transporters gene ABCG2,were tested in SP and MP cells using quantitative real-time reverse transcription polymerase chain reaction(RT-PCR).Western blot was used to show the difference of protein expression between SP and MP cells.Both results show that there was significantly higher protein expression in SP cells than in MP cells.When inoculated into non-obese diabetic/severe combined immunodeficiency(NOD/SCID) mice,SP cells show higher tumorigenesis tendency than MP cells.Conclusions:These results indicate that SP cells possess cancer stem cell properties and prove that SP cells from MKN-45 are gastric cancer stem-like cells. 关键词：肿瘤干细胞细胞系胃癌癌症逆转录聚合酶链反应表征 RT-PCR P细胞

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Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45

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