Community Diversity of Associated Bacteria of Radopholus similis

查看全文 作　　者：Fen [1,2]ZHENG;Meiying [2]FU;Zhixiang [2]ZHAO;Miancai [2]CHEN

机构地区：[1]College of Environment and Plant Protection, Hainan University;[2]Key Laboratory of Plant Diseases and Insect Pests Integrated Control of Hainan Province, Agricultural Environment and Plant Protection Research Institute of Hainan Academy of Agricultural Sciences

出　　处：《Plant Diseases and Pests》索引2012年第3卷第2期,共5页

基　　金：Supported by Special Research Project of Public Sector ( Agriculture )( 200903040) 摘　　要：[Objective] The paper was to reveal the species abundance of associated bacterial community of Radopholus similis, and the diversity of its bacteria. [Methods] Through the construction of 16S rDNA cloning library of bacteria, the positive clone was carried out ARDRA analysis, and the phylogenetic tree was constructed. [Results] OUT (Operational Taxonomic Units) was classified according to sequences similarity of 97%, and the constructed 16S rDNA library of bacteria contained 13 OTUs, belonging to Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria, respectively. The dominant species was Betaproteobacteria, especially Burkholderia was the dominant bacterium. [Conclusions] The diversity of associated bacteria of R. similis was high, and these bacteria may have certain ecological significance to R. similis. 关键词：香蕉穿孔线虫细菌群落群落多样性 DNA克隆 ARDRA 系统发育树序列相似性 DNA文库

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Community Diversity of Associated Bacteria of Radopholus similis

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