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Protective Effects of Quercetin on Cadmium-induced Cytotoxicity in Primary Cultures of Rat Proximal Tubular Cells

查看全文 作　　者：WANG [1]Lin;LIN Shu [2]Qian;HE Yuan [2]Long;LIU [1]Gang;WANG Zhen [1]Yong 高影响力作者 机构地区：[1]College of Animal Science and Veterinary Medicine,Shandong Agricultural University;[2]Institute of Poultry Science,Shandong Academy of Agricultural Sciences高影响力机构 出　　处：《Biomedical and Environmental Sciences》索引2013年第26卷第4期,共10页高影响力期刊 基　　金：supported by the National Nature Science Foundation of China (No. 31101870);Shandong Provincial Natural Science Foundation of China (No.ZR2010CQ014) 摘　　要：Objective To investigate the protective effects of quercetin on cadmium-induced cytotoxicity in primary cultures of rat proximal tubular(rPT)cells.Methods Primary cultures of rPT cells undergoing exponential growth were incubated with 1.0 μg/mL quercetin and/or cadmium(2.5,5.0 μmol/L),in a serum-free medium at 37 ℃ at different time intervals.Commercial kits were used and flow cytometric analyses were performed on rPT cell cultures to assay apoptosis and oxidative stress.Results Exposure of rPT cells to cadmium acetate(2.5,5.0 μmol/L)induced a decrease in cell viability,caused an increase in apoptotic rate and apoptotic morphological changes.Simultaneously,elevation of intracellular reactive oxygen species,malondialdehyde and calcium levels,depletion of mitochondrial membrane potential and intracellular glutathione,and inhibition of Na +,K +-ATPase,Ca 2+-ATPase,glutathione peroxidase(GSH-Px),catalase(CAT),and superoxide dismutase(SOD)activities were revealed during the cadmium exposure of rPT cells.However,simultaneous supplementation with 1 μg/mL quercetin protected rPT cells against cadmium-induced cytotoxicity through inhibiting apoptosis,attenuating lipid peroxidation,renewing mitochondrial function and elevating the intracellular antioxidants(non-enzymatic and enzymic)levels.Conclusion The present study has suggested that quercetin,as a widely distributed dietary antioxidant,contributes potentially to prevent cadmium-induced cytotoxicity in rPT cells. 关 键 词：肾小管上皮细胞 细胞毒性 原代培养 保护作用 醋酸镉 槲皮素 诱导 谷胱甘肽过氧化物酶
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