Jiaweisinisan facilitates neurogenesis in the hippocampus after stress damage

查看全文 作　　者：Lili [1]Wu;Chuanlian [2]Ran;Shukao [3]Liu;Lizhen [4]Liao;Yanling [4]Chen;Hualei [4]Guo;Weikang [4]Wu;Can [1]Yan

机构地区：[1]Department of Basic Theory of Chinese Medicine, Guangzhou University of Chinese Medicine;[2]Department of Traditional Chinese Medicine, Luzhou Medicine College;[3]Institute of Pharmaceutical Research, Yangtze River Pharmaceutical Group Company Limited;[4]Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University

出　　处：《Neural Regeneration Research》索引2013年第8卷第12期,共12页

基　　金：supported by the National NaturalScience Foundation of China,No.30500660 摘　　要：The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 μM corticosterone. Cells were treated with 10% (v/v) Jiaweisinisan drug-containing serum and the corticosterone antagonist RU38486. Results of the 3-(4,5-dimethylthiazol-2-yl)-3,5-di-phenytetrazoliumromide assay showed that both Jiaweisinisan drug-containing serum and RU38486 promoted the proliferation of neural precursor cells after corticosterone exposure. Immunofluorescence detection showed that after Jiaweisinisan drug-containing serum and RU38486 treatment, the 5-bromo-2-deoxyuridine/terminal deoxynucleotidyl transferase dUTP nick end labeling ratio in hippocampal neural precursor cells significantly increased, and the apoptotic rates of glial cells reduced, and neuron-like cell differentiation from neural precursor cells significantly increased. Our experimental findings indicate that Jiaweisinisan promotes hippocampal neurogenesis after stress damage. 关键词：神经前体细胞应激损伤加味无血清培养基生后免疫荧光检测二甲基吡啶脱氧核苷酸

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Jiaweisinisan facilitates neurogenesis in the hippocampus after stress damage

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