AEG1通过激活NF-κB和AP-1诱导肝癌HepG2细胞COX-2表达(英文)

查看全文 作　　者：Huan [1]Deng;Zhenzhen [1]Zhou;Wei [1]Tu;Yujia [1]Xia;Huanjun [1]Huang;De'an [1]Tian

机构地区：[1]Department of Gastroenterology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《The Chinese-German Journal of Clinical Oncology》索引2013年第12卷第6期,共4页

基　　金：Supported by grants from the National Science Foundation of China (No.81070333);the Natural Science Foundation of Hubei Province of China (No.2012FFB02318) 摘　　要：Objective:The aim of this study was to investigate whether astrocyte elevated gene 1 (AEG1) regulates COX-2 expression in human hepatoma HepG2 cells and related pathways involved in this process. Methods:Human hepatoma HepG2 cells were transfected with pcDNA3.1(-)-AEG1 plasmid or psilencer2.0-AEG1-shRNA1 plasmid to up/down-regulate AEG1 expression, pcDNA3.1(-) and psilencer 2.0 empty vector plasmids were transfected respectively as control. Real-time RT-PCR was carried out to measure the expression levels of AEG1 and COX-2 mRNA. The expression levels of AEG1 and COX-2 protein were detected by Western blot. NF-κB signaling was blocked by PDTC, and AP-1 signaling was blocked by curcumin. Results:AEG1 mRNA and protein levels were increased after pcDNA3.1(-)-AEG1 transfection, and decreased after psilencer2.0-AEG1-shRNAs transfection. COX-2 mRNA and protein levels were increased in AEG1-overexpressing cells and decreased in AEG1-knockdown cells. Phosphorylations of p65 and c-jun were up-regulated in AEG1-overexpressing cells. Both PDTC and curcumin reduced COX-2 expression in HepG2 cells with AEG1 overexpression. Conclusion:AEG1-overexpressing and-knockdown HepG2 cells are established successfully. AEG1 could induce COX-2 expression though activating NF-κB and AP-1 in human hepatoma HepG2 cells. 关键词：肿瘤临床治疗化疗

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AEG1通过激活NF-κB和AP-1诱导肝癌HepG2细胞COX-2表达(英文)

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