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MicroRNA-142-3p is frequently upregulated in colorectal cancer and may be involved in the regulation of cell proliferation

查看全文 作　　者：ZHOU [1,2,3]JiaLiang;JIANG [1,4]Zhi;WANG [2,3]ZhengWu;ZOU [2,3]ShiTao;ZHANG [2,3]YunXia;CAI [2,3]Wei;WANG [2,3]MingZhi;XU [2,3]Min;SHI [1]DongTao;CHEN [1]WeiChang 高影响力作者 机构地区：[1]Department of Gastroenterology, the First Affiliated Hospital of Soochow University;[2]Department of Oncology, the Forth Affiliated Hospital of Soochow University;[3]Wuxi No.4 People's Hospital;[4]Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University高影响力机构 出　　处：《Chinese Science Bulletin》索引2013年第58卷第23期,共10页高影响力期刊 摘　　要：MicroRNAs are small single-stranded RNA molecules consisting of approximately 22 nucleotides (nt), and have post-transcriptional regulatory functions. By gene chip screening, we previously showed that miR-142-3p was significantly upregulated in colorectal cancer tissue and was associated with clinicopathological features, compared with matched non-tumor tissue. In this study, we confirmed significant upregulation of miR-142-3p in 60 colorectal cancer samples and three colorectal cancer cell lines by quantitative real-time PCR (qRT-PCR). Using software and network resources, we predicted TCF7 as a target of miR-142-3p, which we confirmed with dual-luciferase assays. By RT-PCR and Western blot analysis, we found miR-142-3p negatively regulates TCF7 expression post-transcriptionally. CCK8 assays and growth curves indicated that overexpression of miR-142-3p in SW480 colorectal cancer cells potently inhibited cell proliferation in vitro. The expression of TCF7 mRNA and protein was upregulated in both colorectal cancer tissues and colorectal cancer cell lines, closely correlating with its function as an oncogene in promoting tumor cell proliferation and inhibiting apoptosis. With TCF7 as a direct target, our results suggested that miR-142-3p may be involved in the regulation of cell proliferation in colorectal cancer. 关 键 词：细胞增殖 大肠癌 实时定量RT-PCR MICRORNA 实时定量PCR BLOT分析 肿瘤组织 核苷酸组成
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