Zinc Fingers, TAL Effectors, or Cas9-Based DNA Binding Proteins: What's Best for Targeting Desired Genome Loci?

查看全文 作　　者：Annett Strauβ Thomas [1]Lahaye

机构地区：[1]Faculty of Biology, Institute of Genetics, Ludwig-Maximilians-University Munich, Grolβhaderner Straβe 2-4, D-82152 Martinsried, Germany

出　　处：《Molecular Plant》索引2013年第6卷第5期,共4页

摘　　要：ZINC FINGER-AND TAL EFFECTORBASED DNA BINDING PROTEINS Programmable DNA binding proteins (PDPs) that selectively bind user-defined nucleotide sequences can navigate protein domains to any desired target within the genome.Different functional domains can be fused to PDPs to achieve (1) activation or repression of transcription,(2) nicking or cleavage of DNA,or (3) modification or removal of epigenetic marks at the targeted genome spot.Given their broad applicability,the development of highly specific,easy-to-use PDPs has been a holy grail of biotechnology since the determination of the 3-D structure of zinc fingers (ZFs) bound to DNA in 1991 (Pavletich and Pabo,1991). 关键词：DNA结合蛋白基因组 TAL 锌指期望基础蛋白质结构域用户自定义

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Zinc Fingers, TAL Effectors, or Cas9-Based DNA Binding Proteins: What's Best for Targeting Desired Genome Loci?

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