Two outward potassium current types are expressed during the neural differentiation of neural stem cells

查看全文 作　　者：Ruiying [1]Bai;Guowei [2]Gao;Ying [1,3]Xing;Hong [4]Xue

机构地区：[1]Department of Physiology and Neurobiology,Xinxiang Medical University;[2]Department of Radiotherapy,Center Hospital of Xinxiang;[3]Stem Cell Research Center,Zhengzhou University;[4]Basic Medical Sciences of Henan University of Traditional Chinese Medicine

出　　处：《Neural Regeneration Research》索引2013年第8卷第28期,共10页

基　　金：supported by the National Natural Science Foundation of China,No.31000514;the Scientific Research Project for Talent with High Education of Xinxiang Medical University,No.2007502002 摘　　要：The electrophysiological properties of potassium ion channels are regarded as a basic index for determining the functional differentiation of neural stem cells.In this study,neural stem cells from the hippocampus of newborn rats were induced to differentiate with neurotrophic growth factor,and the electrophysiological properties of the voltage-gated potassium ion channels were observed.Immunofluorescence staining showed that the rapidly proliferating neural stem cells formed spheres in vitro that expressed high levels of nestin.The differentiated neurons were shown to express neuron-specific enolase.Flow cytometric analysis revealed that the neural stem cells were actively dividing and the percentage of cells in the S+G2/M phase was high.However,the ratio of cells in the S+G2/M phase decreased obviously as differentiation proceeded.Whole-cell patch-clamp recordings revealed apparent changes in potassium ion currents as the neurons differentiated.The potassium ion currents consisted of one transient outward potassium ion current and one delayed rectifier potassium ion current,which were blocked by 4-aminopyridine and tetraethylammonium,respectively.The experimental findings indicate that neural stem cells from newborn rat hippocampus could be cultured and induced to differentiate into functional neurons under defined conditions in vitro.The differentiated neurons expressed two types of outward potassium ion currents similar to those of mature neurons in vivo. 关键词：神经干细胞分化过程钾电流神经元特异性烯醇化酶类型钾离子通道流式细胞仪分析免疫荧光染色

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Two outward potassium current types are expressed during the neural differentiation of neural stem cells

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