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Effects of tyrosine kinase inhibitor E7080 and eNOS inhibitor L-NIO on colorectal cancer alone and in combination

查看全文 作　　者：Ahmet [1]Altun;Tijen Kaya [1]Temiz;Ezgi [1]Balc;Zübeyde Ak n [2]Polat;Mustafa [3]Turan 高影响力作者 机构地区：[1]Department of Pharmacology,Cumhuriyet University School of Medicine;[2]Department of Parasitology,Cumhuriyet University School of Medicine;[3]Department of General Surgery,Cumhuriyet University School of Medicine高影响力机构 出　　处：《Chinese Journal of Cancer Research》索引2013年第25卷第5期,共13页高影响力期刊 摘　　要：Objective:To investigate the effects of E7080 and N5-(1-iminoethyl)-L-ornithine dihydrochloride(L-NIO)on colorectal cancer alone and in combination.Methods:HT29 colorectal cancer cell line from Sap Institute was used.Real-time cell analysis(xCELLigence system)was performed to determine the effects of E7080 and L-NIO on colorectal cell proliferation.While apoptosis was determined with Annexin V staining,and the effect of agents on angiogenesis was determined with chorioallantoic membrane(CAM)model.Results:We found that E7080 has a strong antiproliferative effect with an half maximum inhibition of concentration(IC50)value of 5.60×10–8mol/L.Also it has been observed that E7080 showed antiangiogenic and apoptotic effects on HT29 colorectal cancer cells.Antiangiogenic scores of E7080 were 1.2,1.0 and 0.6for 100,10 and 1 nmol/L E7080 concentrations,respectively.Furthermore,apoptosis has been detected in71%of HT29 colorectal cancer cells after administration of 100 nmol/L E7080 which may indicate strong apoptotic effect.Meanwhile administration of L-NIO alone did not show any effect,but the combination of E7080 with L-NIO increased the antiproliferative,antiangiogenic and apoptotic effects of E7080.Conclusions:Results of this study indicate that E7080 may be a good choice in treatment of colorectal tumors.Furthermore the increased effects of E7080 when combined with L-NIO raise the possibility to use a lower dose of E7080 and therefore avoid/minimize the side effects observed with E7080. 关 键 词：酪氨酸激酶抑制剂 结直肠癌 NOS抑制剂 结肠 抗血管生成 细胞凋亡 细胞增殖 L-鸟氨酸
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