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Isolation and Sequence Analysis of NSP2, ORF3 and ORF5 of PRRSV XH-GD Strain from Guangdong Province

查看全文 作　　者：Zongxi [1,2]CAO;Quanhui [2]PAN;Zhemin [1]LIN;Liuwu [2]KONG;Yimin [2]HE;Guihong [2]ZHANG 高影响力作者 机构地区：[1]Hainan Provincial Key Laboratory of Tropical Animal Reproduction and Breeding and Veterinary Medicine,Institute of Animal Husbandry and Veterinary Medicine,Hainan Academy of Agricultural Sciences;[2]MOA Key Laboratory for Animal Vaccine Development,South China Agricultural University高影响力机构 出　　处：《Agricultural Biotechnology》索引2014年第3卷第1期,共5页高影响力期刊 基　　金：Supported by National Natural Science Foundation of China(31272564);Joint Fund of NSFC-Guangdong of China(U0931003);China Agriculture Research System(CARS-36);Hainan Provincial Research Foundation for public institutions(Qiong Cai Yu[2013]No.131) 摘　　要：The diseased samples collected from Guangdong Province were inoculated to Marc-145 cells,PRRSV-specific cytopathogenic effects( CPE) such as cell aggregation,cell shrinkage and cell exfoliation were observed and a PRRSV strain XH-GD was obtained. By using primers designed in accordance with the published gene sequences of PRRSV in GenBank,NSP2,ORF3 and ORF5 fragments were amplified by reverse transcription polymerase chain reaction and analyzed by sequence alignment. The results demonstrated that PRRSV strain XH-GD belonged to the North American genotype and the NSP2 gene contained discontinuous deletions of 30 amino acids. NSP2,ORF3 and ORF5 genes of XH-GD shared 83. 3%- 98. 9%,88. 6%- 99. 2% and 88. 1%- 99. 2% nucleotide homology,and76. 8%- 98. 3%,83. 7%- 98. 8% and 88. 1%- 99. 2% amino acid homology with HUB1,NX06,BJsy06,VR-2332,HB-1( sh) 2002,HB-2( sh) 2002,CH-la and RespPRRS MLV,respectively; however,NSP2,ORF3 and ORF5 genes of XH-GD shared only 52. 9%,65. 0% and 64. 3% nucleotide homology,and31. 3%,57. 5% and 58. 9% amino acid homology with LV,respectively. Phylogenetic analysis revealed that XH-GD had a closer relationship with HUB1,NX06,BJsy06,VR-2332,HB-1( sh) 2002,HB-2( sh) 2002,CH-la and RespPRRS MLV strains compared with LV strain. 关 键 词：ORF5基因 PRRSV 序列分析 广东省 致细胞病变效应 逆转录聚合酶链反应 氨基酸同源性 核苷酸同源性
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