Determination of Berberine Hydrochloride Content in Qingwen Baidu Granule by HPLC

查看全文 作　　者：Guo [1,2]Shijin;Wang [1,2]Yanping;Fu [1]Shijun;Zhang [1,2]Zhimei;Xu [1,2]Qianqian;Shen [1,2]Zhiqiang

机构地区：[1]Shandong Binzhou Animal Science and Veterinary Medicine Academy;[2]Shandong Lvdu Ante Veterinary Drug Co. ,Ltd.

出　　处：《Animal Husbandry and Feed Science》索引2014年第6卷第1期,共4页

基　　金：Supported by Technology Innovation Project of Shandong Province(201210916001) 摘　　要：The paper was to establish a simple and sensitive HPLC method for determination of berberine hydrochloride content in Qingwen Baidu granule.The HPLC system consisted of Stable Bond- C18 column(4.6×150 mm,5μm) and with a mobile phase of acetonitrile- 0.05mol / L potassium dihydrogen phosphate solution(50∶50,v∶v) mixture.Berberine hydrochloride was detected at the wavelength of 345 nm,with a flow rate of 1.0 mL / min and a column temperature of30 ℃.The excipients and solvents in the granule could be well separated from the drug under such a designated chromatogram condition and did not interfere with the assay.A good linear relationship was found between peak area and the concentration of berberine hydrochloride in the range of 16.0- 48.0 μg / mL.The average recovery of berberine hydrochloride was 99.09%.The established approach was specific,accurate,reliable,prompt,sensitive and applicable,and could be used to control the quality of Qingwen Baidu granule. 关键词：盐酸小檗碱高效液相色谱法颗粒百度测定黄连素 HPLC系统 HPLC法

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Determination of Berberine Hydrochloride Content in Qingwen Baidu Granule by HPLC

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