High-throughput sequencing exclusively identified a novel Torque teno virus genotype in serum of a patient with fatal fever

查看全文 作　　者：Zhiqiang [1]Mi;Xin [2]Yuan;Guangqian [1]Pei;Wei [1]Wang;Xiaoping [1]An;Zhiyi [1]Zhang;Yong [1]Huang;Fan [1,2,3]Peng;Shasha [1]Li;Changqing [2]Bai;Yigang [1]Tong

机构地区：[1]State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology;[2]Department of Respiratory Medicine, PLA Hospital 307;[3]The Third Xiangya Hospital of Central South University

出　　处：《Virologica Sinica》索引2014年第29卷第2期,共7页

基　　金：supported by a grant from the National Natural Science Foundation of China (No. 81072350);the National Hi-Tech Research and Development (863) Program of China (No. 2012AA022-003);the China Mega-Project on Major Drug Development (No. 2011ZX09401-023);the China Mega-Project on Infectious Disease Prevention (No. 2013ZX10004-605, No. 2013ZX10004-607, No. 2013ZX10004-217, and No. 2011ZX10004-001) ;the State Key Laboratory of Pathogen and BioSecurity Program (No. SKLPBS1113) 摘　　要：Torque teno virus(TTV) has been found to be prevalent world-wide in healthy populations and in patients with various diseases, but its etiological role has not yet been determined. Using high-throughput unbiased sequencing to screen for viruses in the serum of a patient with persistent high fever who died of suspected viral infection and prolonged weakness, we identified the complete genome sequence of a TTV(isolate Hebei-1). The genome of TTV-Hebei-1 is 3649 bp in length, encoding four putative open reading frames, and it has a G+C content of 49%. Genomic comparison and a BLASTN search revealed that the assembled genome of TTV-Hebei-1 represented a novel isolate, with a genome sequence that was highly heterologous to the sequences of other reported TTV strains. A phylogenetic tree constructed using the complete genome sequence showed that TTV-Hebei-1 and an uncharacterized Taiwan Residents strain, TW53A37, constitute a new TTV genotype. The patient was strongly suspected of carrying a viral infection and died eventually without any other possible causes being apparent. No virus other than the novel TTV was identified in his serum sample. Although a direct causal link between the novel TTV genotype infection and the patient's disease could not be confirmed, the findings suggest that surveillance of this novel TTV genotype is necessary and that its role in disease deserves to be explored. 关键词：病毒感染血清样品基因型高通量测序全基因组序列发烧病转矩

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High-throughput sequencing exclusively identified a novel Torque teno virus genotype in serum of a patient with fatal fever

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