Effects of feeding tyrosine and phenylalanine on the accumulation of phenylethanoid glycosides to Cistanche deserticola cell suspension culture

查看全文 作　　者：HU Gao-[1,2,3]Sheng;JIA Jing-[1,2,3]Ming;Doh Hoon [2,3,4]Kim

机构地区：[1]School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University;[2]Joint Laboratory of Shenyang Pharmaceutical University;[3]Dong-A University;[4]College of Life Science and Natural Resources, Dong-A University

出　　处：《Chinese Journal of Natural Medicines》索引2014年第12卷第5期,共6页

基　　金：funded by Dong A University 摘　　要：AIM: To investigate the effects of feeding phenylalanine(Phe) and tyrosine(Tyr) on the accumulation of total phenolic compounds and four phenylethanoid glycosides(PeGs) to a cell suspension culture of the parasitic plant Cistanche deserticola. METHOD: A cell suspension culture of C. deserticola was established and precursors of different concentrations were fed. In each group, the cell was sampled at the 24th day after inoculation. The content of total phenolic compounds and four PeGs compounds were determined using the Folin-Ciocalteu method and an HPLC method, respectively. RESULTS: In the Phe fed cells, the maximum PeGs yield was achieved when Phe was fed at 1.5 mmol·L-1 and the yield reached 1.13 times the control cell concentration. In the Tyr fed cells, the maximum yield of PeGs was 1.60 times of control when 0.75 mmol·L-1 Tyr was fed to the cells. Furthermore, it was found that the salidroside yield was 4.01 times of control group when 5 mmol·L-1 Tyr was fed. CONCLUSION: Tyr is a better precursor for PeGs accumulation compared with Phe, and the rate limiting enzymes might be involved in the Tyr branch. 关键词：药理学天然药物性质实验药理学

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Effects of feeding tyrosine and phenylalanine on the accumulation of phenylethanoid glycosides to Cistanche deserticola cell suspension culture

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