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Aqueous extract of Taxus Chinensis (Pilger) Rehd inhibits lung carcinoma A549 cells through the epidermal growth factor receptor/mitogen-activated protein kinase pathway in vitro and in vivo

查看全文 作　　者：Qijin [1]Shu;Minhe [1]Shen;Binbin [1]Wang;Qingli [2,3]Cui;Xiaoying [2,4]Zhou;Luming [2,5]Zhu 高影响力作者 机构地区：[1]Department of Oncology, Zhejiang Provincial Hospital of Traditional Chinese Medicine;[2]Department of Oncology, the First Clinical Medical College and the First Affiliated Hospital of Zhejiang Chinese Medical University;[3]Department of Oncology, Zhengzhou Cancer Hospital;[4]Department of Oncology, Tongxiang First People's Hospital;[5]Department of Surgery, Haining Hospital of Traditional Chinese Medicine高影响力机构 出　　处：《Journal of Traditional Chinese Medicine》索引2014年第34卷第3期,共9页高影响力期刊 基　　金：Supported by Natural Science Foundation of Zhejiang Province(No.Y2081051);Science and Technology Planning Key Project of Zhejiang Province(No.2009ZA003) 摘　　要：OBJECTIVE:To explore the anticancer mechanismof aqueous extract of Taxus Chinensis (Pilger) Rehd (AETC).METHODS: The serum pharmacological method was used to avoid interference from administration of the crude medicinal herbs. Eight purebred New Zealand rabbits were used for preparation of serum containing various concentrations of AETC. Forty-eightBalb/c-nu mice were used for in vivo experiments. The effects of serum containing AETC on the proliferation of A549 cells and expression levels of the epidermal growth factor receptor/mitogen-activated protein kinase(EGFR/MAPK) pathway-related proteins in vitro were investigated. Additionally, the effects on the growth of A549 xenografts in nudemice,and expression levels of the EGFR/MAPK pathway-related proteins in the xenografts,were investigated.RESULTS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay revealed that the serum containing AETC significantly decreased the viability of A549 cells in a dose-dependent manner.Western blot showed that the serum containing various concentrations of AETC strongly reduced the levels of phospho-Jun N-terminal kinase(p-JNK) and phospho-extracellular signal-regulated kinase1/2(ERK1/2) while it increased the level of p-p38. However, no significant effects on the expression levels of JNK, ERK1/2, and p38 MAPK were found. In addition, an anticancer effect from AETC was observed invivo in the Balb/c-nu mice bearing A549xenografts.CONCLUSION: AETC has significant effects on the growth of A549 xenografts and on the activity of the EGFR/MAPK pathway. Therefore, AETC may be beneficial in lung carcinoma treatment. 关 键 词：表皮生长因子受体 蛋白激酶途径 A549细胞 水提取物 体内实验 红豆杉 皮下埋植 体外
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