Progress in TILLING as a tool for functional genomics and improvement of crops

查看全文 作　　者：Liang [1]Chen;Liugen [1]Hao;Martin A. J. [2]Parry;Andrew L. [2]Phillips;Yin-Gang [1,3]Hu

机构地区：[1]State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University;[2]Department of Plant Biology and Crop Science, Rothamsted Research;[3]Institute of Water Saving Agriculture in Arid Regions of China, Northwest A&F University

出　　处：《Journal of Integrative Plant Biology》索引2014年第56卷第5期,共19页

基　　金：supported by the sub‐project of the 863 Program(2011AA100504,2013AA102902)of the Ministry of Science and Technology;the key project of Chinese Universities Scientific Fund;Northwest A&F University(ZD2012002);the China 111 Project(B12007),P.R.China;the ACIAR Project(CIM/2005/111)of Australia.ALP;supported by the Biotechnology and Biological Sciences Research Council(BBSRC)of the UK under the 20:20 WheatInstitute Strategic Programme 摘　　要：Food security is a global concern and substantial yield increases in crops are required to feed the growing world population. Mutagenesis is an important tool in crop improvement and is free of the regulatory restrictions imposed on genetically modified organisms. Targeting Induced Local Lesions in Genomes(TILLING), which combines traditional chemical mutagenesis with high‐throughput genome‐wide screening for point mutations in desired genes, offers a powerful way to create novel mutant alleles for both functional genomics and improvement of crops. TILLING is generally applicable to genomes whether small or large, diploid or evenallohexaploid, and shows great potential to address the major challenge of linking sequence information to the function of genes and to modulate key traits for plant breeding. TILLING has been successfully applied in many crop species and recent progress in TILLING is summarized below, especially on the developments in mutation detection technology, application of TILLING in gene functional studies and crop breeding. The potential of TILLING/EcoTILLING for functional genetics and crop improvement is also discussed. Furthermore, a small‐scale forward strategy including backcross and selfing was conducted to release the potential mutant phenotypes masked in M2(or M3) plants. 关键词：功能基因组学农作物工具突变等位基因作物改良植物育种转基因生物点突变

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Progress in TILLING as a tool for functional genomics and improvement of crops

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