Studies on Human Fetal Lens Crystallins Under Oxidative Stress and Protective Effects of Tea Polyphenols

查看全文 作　　者：Hui Xiang, Suhua Pan, Shaozhen LiGuangdong Provincial Corps Hospital, Chinese People’ s Armed Police Forces, Guangzhou 510010, China Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China 出　　处：《眼科学报》索引1998年第14卷第3期,共6页 摘　　要：Purpose: To investigate the oxidative modification of water-soluble crystallins of human fetal lens with H2O2 and fourteen metal ions with or without EDTA. Tea-polyphenols (TP) was added to above solutions in order to testing their antioxidative abilities. Methods: The experiments were performed at 37℃ with final concentration of 2.5mg/ml protein, 0.1 mM metal ions, 0.3 mM EDTA and 1.0 mM H2O2. Then the TP was added to the solution with CuSO4 and H2O2, after 5 or 24 hours, the crystallins were analysed with SDS-PAGE and IEF.Results : There were marked oxidative modifications of lens protein in H2O2 and copper without EDTA. In SDS-PAGE patterns, we found an increase in those species above of bands higher than 30 kD and some diffuse bands from 30 to 17 kD after 5 hours. In IEF patterns, there were a general increase on acidity with loss of the more basic species. When the TP was added, there was not any difference with control group.Conclusion: The results indicate that exposure of water-soluble 关 键 词：人 胎儿 晶状体 晶体蛋白 氧化变性 抗氧化作用 多酚