Effect of Ginkgo biloba leaf extract on cerebral cortex amino acid levels in cerebral ischemia model rats

查看全文 作　　者：Cui [1,2]Yiran;Wu [2]Hongwei;Liu [2]Mengting;Qin [3]Haijiao;Liu [4]Xin;Yang [2]Hongjun

机构地区：[1]Beijing Hospital of Traditional Chinese Medicine,Capital Medical University;[2]Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;[3]College of Science, Central South University of Forestry and Technology;[4]School of Chinese Materia Medica, Beijing University of Chinese Medicine

出　　处：《Journal of Traditional Chinese Medicine》索引2018年第38卷第5期,共9页

基　　金：supported by the Key Program of National Natural Science Foundation of China (81330086);the General Program of National Natural Science Foundation of China (81573726, 81403210);the Fundamental Research Funds for the Central Universities (No. 2017-JYB-JS-054) 摘　　要：OBJECTIVE: To investigate the effect of Ginkgo biloba leaf extract on amino acid levels in the cerebral cortex of cerebral ischemia model rats induced by middle cerebral artery occlusion(MCAO).METHODS: A rat model of cerebral ischemia was established by MCAO. Male rats were divided into a negative control group(Control), a sham-operated group(Sham), an ischemic group(MCAO), and an ischemic group treated with Ginkgo biloba leaf extract(MCAO_D). All groups were divided into two subgroups with occlusion times of 12 and 24 h, respectively. The levels of 18 endogenous amino acids in the cerebral cortex were quantified by triple quadrupole-liquid chromatography-mass spectrometry.RESULTS: Compared with the MCAO group, behavioral performance, neurological deficit score, and cerebral infarct volume were significantly improved in the MCAO_D group(P < 0.05, P < 0.01). Compared with the sham group, the levels of 17 amino acids in the cerebral cortex were markedly changed in the MCAO group. The levels of Alanine(Ala), Isoleucine(Ile), Glutamic acid(Glu), Serine(Ser), Valine(Val), Phenylalanine(Phe), Proline(Pro),Threonine(Thr), Lysine(Lys), Tyrosine(Tyr), Hydroxyproline(Hyp), Arginine(Arg), Leucine(Leu),Tryptophan(Trp), and Glycine(Gly) were increased(P < 0.001, P < 0.05), while levels of Gln and Tau were decreased(P < 0.001, P < 0.05). Compared with the MCAO group, Ginkgo biloba extract treatment in the MCAO_D group significantly down-regulated the levels of 11 amino acids, especially those of Arg, Thr, and Ser in 12 or 24 h.CONCLUSION: Injection of Ginkgo biloba leaf extract has a therapeutic effect on model rats with MCAO-induced cerebral ischemia by acting on amino acids in the cerebral cortex. This effect might be associated with the regulation of amino acid metabolism in the cerebral cortex. 关键词：Cerebral ISCHEMIA GINKGO biloba leaf extract AMINO ACIDS Metabolism

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Effect of Ginkgo biloba leaf extract on cerebral cortex amino acid levels in cerebral ischemia model rats

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