Manipulating mRNA splicing by base editing in plants

查看全文 作　　者：Chenxiao [1,2]Xue;Huawei [1]Zhang;Qiupeng [1,2]Lin;Rong [1,2,3]Fan;Caixia [1,2]Gao

机构地区：[1]State Key Laboratory of Plant Cell and Chromosome Engineering,Center for Genome Editing,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China;[2]University of Chinese Academy of Sciences,Beijing 100049,China;[3]Hebei Laboratory of Agricultural Water-Saving,Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang 050021,China

出　　处：《Science China(Life Sciences)》索引2018年第61卷第11期,共8页

基　　金：supported by grants from the National Key Research and Development Program of China (2016YFD0101804);the National Natural Science Foundation of China (31788103 and 31420103912);as well as the Chinese Academy of Sciences (QYZDY-SSWSMC030 and GJHZ1602) 摘　　要：Precursor-mRNAs(pre-mRNA) can be processed into one or more mature m RNA isoforms through constitutive or alternative splicing pathways. Constitutive splicing of pre-mRNA plays critical roles in gene expressional regulation, such as intronmediated enhancement(IME), whereas alternative splicing(AS) dramatically increases the protein diversity and gene functional regulation. However, the unavailability of mutants for individual spliced isoforms in plants has been a major limitation in studying the function of mRNA splicing. Here, we describe an efficient tool for manipulating the splicing of plant genes. Using a Cas9-directed base editor, we converted the 5′ splice sites in four Arabidopsis genes from the activated GT form to the inactive AT form. Silencing the AS of HAB 1.1(encoding a type 2 C phosphatase) validated its function in abscisic acid signaling, while perturbing the AS of RS31 A revealed its functional involvement in plant response to genotoxic treatment for the first time. Lastly,altering the constitutive splicing of Act2 via base editing facilitated the analysis of IME. This strategy provides an efficient tool for investigating the function and regulation of gene splicing in plants and other eukaryotes. 关键词：BASE EDITING SPLICING INTRON RETENTION ALTERNATIVE 5'splicing site

相关文献

参考文献（24）
引证文献（20）
耦合文献（155）

参考文献(24)

引证文献(20)

耦合文献(155)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Manipulating mRNA splicing by base editing in plants

参考文献(24)

引证文献(20)

耦合文献(155)

用户提示