Culture and Identification of Myoblasts Isolated from Duck Embryos

查看全文 作　　者：Yanju [1]SHAN;Jingting [1]SHU;Chi [1]SONG;Yan [1]HU;Jian [2]CHEN;Huifang [1]LI

机构地区：[1]Institute of Poultry Science, Chinese Academy of Agricultural Sciences;[2]Jiangsu Tengdayuang Co. Ltd

出　　处：《Agricultural Science & Technology》索引2014年第15卷第8期,共4页

基　　金：Supported by the National Natural Science Foundation of China(31172194);Science and Technology Support Program of Jiangsu Province(BE2014362) 摘　　要：[Objective] Using embryonic myoblasts to research the formation and developmental mechanisms of skeletal muscle is becoming a research hotspot. This study aimed to establish a method of isolation, culture and identification of myoblasts in duck embryos. [Method] Pectoral and leg muscle samples were isolated from the embryos of Gaoyou duck at 13 d of hatching, then disassociated with collagenase and trypsin and purified via differential adhesion. The isolated cells were cultured in vitro and detected for the expression of Pax7 protein using immunofluorescence technique. [Result] Myoblasts were obtained successfully both from pectoral and leg muscles in duck embryos and these cells proliferated strongly and differentiated well. Immunofluorescence staining showed that more than 95% cells could express Pax7 protein. [Conclusion] In summary, we report the successful establishment of a complete system for the isolation, purification, identification and culture of myoblasts from duck embryos. 关键词：细胞培养骨骼肌分离胚胎鸭胚鉴定成肌细胞胰蛋白酶

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Culture and Identification of Myoblasts Isolated from Duck Embryos

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