Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway

查看全文 作　　者：XIONG [1]Lu;SUN Cheng [1]Feng;ZHANG [1]Jing;GAO Ya [1]Bing;WANG Li [1]Feng;ZUO Hong [1]Yan;WANG Shui [1]Ming;ZHOU Hong [1]Mei;XU Xin [1]Ping;DONG [1]Ji;YAO Bin [1]Wei;ZHAO [1]Li;PENG Rui [1]Yun

机构地区：[1]Beijing Institute of Radiation Medicine

出　　处：《Biomedical and Environmental Sciences》索引2015年第28卷第1期,共12页

基　　金：supported by the National Natural Science Foundation of China(No.81172620) 摘　　要：Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor(NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment. Methods 48 male Wistar rats were exposed to 30 m W/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 m W/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated. Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits(NR1, NR2 A, and NR2B) and related signaling molecules(Ca2+/calmodulin-dependent kinase II gamma and phosphorylated c AMP-response element binding protein) were examined. Conclusion 30 m W/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity. 关键词：WISTAR大鼠 NMDA受体 PC12细胞突触可塑性微波辐射海马结构信号通路辐射损害

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Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway

参考文献(42)

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