Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival

查看全文 作　　者：Dmitriy Sergeevich [1]Kobyakov;Ashot Merudzhanovich [2]Avdalyan;Aleksandr Fedorovich [2]Lazarev;Elena Leonidovna [3]Lushnikova;Lev Moiseevich [3]Nepomnyashchikh 机构地区：[1]Budget Institution, Kogalym City Hospital;[2]Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N.Blokhin Cancer Research Center;[3]Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences 出　　处：《Cancer Biology & Medicine》索引2014年第11卷第4期,共6页 摘　　要：Objective:To evaluate the relation between argyrophilic nucleolar organizer region(Ag NOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer(NSCLC).Methods:A total of 207 surgical specimens diagnosed as NSCLC were included in this study.Double-staining procedures were performed using antigen Ki-67(clone MIB-1)and silver nitrate by immunohistochemical and Ag NOR-staining methods.Results:The Ag NOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM(tumor,node,and metastasis)system.The survival of patients with small Ag NOR area in MIB-1-positive cells is better than that of patients with large Ag NOR area.Molecular,biological(Ag NOR area in MIB-1-positive cells),and clinicopathological(greatest tumor dimension,metastases to regional lymph nodes,histology,and differentiation)parameters are independent prognostic factors of NSCLC.Conclusion:The Ag NOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC. 关 键 词：非小细胞肺癌 阳性细胞 临床意义 核仁组成区 嗜银蛋白 AGNOR 免疫组织化学 临床病理