Proline with or without Hydroxyproline Influences Collagen Concentration and Regulates Prolyl 4-Hydroxylase α(I) Gene Expression in Juvenile Turbot(Scophthalmus maximus L.)

查看全文 作　　者：ZHANG [1]Kaikai;MAI [1]Kangsen;XU [1]Wei;ZHOU [1]Huihui;LIUFU [1]Zhiguo;ZHANG [1]Yanjiao;PENG [1]Mo;AI [1]Qinghui

机构地区：[1]The Key Laboratory of Mariculture (Ministry of Education of China), Ocean University of China

出　　处：《Journal of Ocean University of China》索引2015年第14卷第3期,共8页

基　　金：financially supported by the China Agriculture Research System (CARS-50-G08);Agricultural Scientific and Technological Achievements into Capital (2010GB23600673);the National Natural Science Foundation of China (Grant Nos. 31072222 and 30901 108) 摘　　要：This study was conducted to investigate the effect of dietary proline(Pro), and Pro and hydroxyproline(Hyp) in combination on the growth performance, total Hyp and collagen concentrations of tissues, and prolyl 4-hydroxylase α(I)(P4H α(I)) gene expression in juvenile turbot feeding high plant protein diets. A diet containing 50% crude protein and 12% crude lipid was formulated as the basal and control, on which other two protein and lipid contents identical experimental diets were formulated by supplementing the basal with either 0.75% Pro(Pro-0.75) or 0.75% Pro and 0.75% Hyp(Pro+Hyp). Four groups of fish in indoor seawater recirculating systems, 35 individuals each, were fed twice a day to apparent satiation for 10 weeks. The results showed that dietary Pro and Hyp supplementation had no significant effect on growth performance and feed utilization of juvenile turbot(P > 0.05). Total Hyp and collagen concentrations in muscle were significantly increased when dietary Pro and Hyp increased(P < 0.05), and fish fed diet Pro+Hyp showed significantly higher free Hyp content in plasma than those fed other diets(P < 0.05). The expression of P4 H α(I) gene in liver and muscle was significantly up regulated in fish fed diet Pro-0.75 in comparison with control(P < 0.05); however the gene was significantly down regulated in fish fed diet Pro+Hyp in muscle in comparison with fish fed diet Pro-0.75(P < 0.05). It can be concluded that supplement of crystal L-Pro and L-Hyp to high plant protein diets did not show positive effects on growth performance of juvenile turbot, but enhanced total collagen concentrations in muscle. 关键词：羟脯氨酸幼鱼饲养基因表达胶原含量大菱鲆羟化酶氨酰植物蛋白饲料

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Proline with or without Hydroxyproline Influences Collagen Concentration and Regulates Prolyl 4-Hydroxylase α(I) Gene Expression in Juvenile Turbot(Scophthalmus maximus L.)

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