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Effects of 5-Aminolevulinic Acid on the Content of Total Flavonoids and Expression of CHS and CHI Genes in Young Apples

查看全文 作　　者：Lei [1]CHEN;Yurong [1]GUO;Xiaorui [1]ZHANG;Ruifang [1]MI 高影响力作者 机构地区：[1]College of Food Engineering and Nutritional Science,Shaanxi Normal University高影响力机构 出　　处：《Agricultural Biotechnology》索引2015年第4卷第3期,共4页高影响力期刊 基　　金：Supported by Earmarked Fund for Modern Agro-industry Technology Research System of China(GK661001) 摘　　要：[Objective] This study aimed to investigate the effects of 5-aminolevulinic acid( ALA) on the content of total flavonoids and relative expression levels of chalcone sythase( CHS) and chalcone isomerase( CHI) genes in young apples to determine the appropriate ALA concentration and processing time. [Method]Before thinning,young apples were treated with 0( CK),100,200,300 and 400 mg / L ALA. At 12 d after ALA treatment,the content of total flavonoids in young apples was determined by ultraviolet spectrophotometry. The relative expression levels of CHS and CHI genes in young apples were determined by q PCR. [Result]When ALA concentration was 0- 300 mg / L,the content of total flavonoids and relative expression levels of CHS and CHI genes in young apples were improved with the increase of ALA concentration. As ALA concentration rose to 400 mg / L,various indicators showed a downward trend. Moreover,the content of total flavonoids and relative expression levels of CHS and CHI genes in young apples treated with different concentrations of ALA were improved significantly,which reached the maximum at 9 d and declined since 12 d. [Conclusion] Compared with CK,spraying young apples with 300 mg / L ALA at 6- 9 d before thinning was conducive to improving the content of flavonoids in thinned young apples. 关 键 词：5-氨基乙酰丙酸 小苹果 I基因 CHS 总黄酮 表达量 分光光度法测定 ALA
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