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^(99m)TC-Methylene diphosphonate uptake at injury site correlates with osteoblast differentiation and mineralization during bone healing in mice

查看全文 作　　者：Zhendong A [1,2]Zhong;Anderson [3]Peck;Shihong [3]Li;Jeff Van [3]Oss;John [1]Snider;Casey J [1]Droscha;Tingtung A [3]Chang;Bart O [1]Williams 高影响力作者 机构地区：[1]Center for Skeletal Disease Research, Van Andel Research Institute;[2]Department of Internal Medicine, Center for Musculoskeletal Health, UC Davis Medical Center;[3]Small Animal Imaging Facility, Van Andel Research Institute高影响力机构 出　　处：《Bone Research》索引2015年第3卷第2期,共8页高影响力期刊 基　　金：supported by the Van Andel Research Institute;by a grant to BOW from the NIH/NIAMS (AR053293) 摘　　要：99m Tc-Methylene diphosphonate(99m Tc-MDP) is widely used in clinical settings to detect bone abnormalities.However, the mechanism of99 m Tc-MDP uptake in bone is not well elucidated. In this study, we utilized a mouse tibia injury model, single-photon emission computed tomography(gamma scintigraphy or SPECT),ex vivo micro-computed tomography, and histology to monitor99 m Tc-MDP uptake in injury sites during skeletal healing. In an ex vivo culture system, calvarial cells were differentiated into osteoblasts with osteogenic medium, pulsed with99 m Tc-MDP at different time points, and quantitated for99 m Tc-MDP uptake with a gamma counter. We demonstrated that99 m Tc-MDP uptake in the injury sites corresponded to osteoblast generation in those sites throughout the healing process. The99 m Tc-MDP uptake within the injury sites peaked on day 7 post-injury, while the injury sites were occupied by mature osteoblasts also starting from day 7.99 m Tc-MDP uptake started to decrease 14 days post-surgery, when we observed the highest level of bony tissue in the injury sites. We also found that99 m Tc-MDP uptake was associated with osteoblast maturation and mineralization in vitro. This study provides direct and biological evidence for99 m Tc-MDP uptake in osteoblasts during bone healing in vivo and in vitro. 关 键 词：成骨细胞分化 损伤部位 愈合过程 二膦酸盐 P吸收 亚甲基 矿化 小鼠
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