Modulation of Gene Expression in Key Survival Pathways During Daily Torpor in the Gray Mouse Lemur, Microcebus murinus

查看全文 作　　者：Kyle [1,2]K.Biggar;Cheng-Wei [1,3]Wu;Shannon [1,4]N.Tessier;Jing [1,5]Zhang;Fabien [6]Pifferi;Martine [6]Perret;Kenneth [1]B.Storey 机构地区：[1]Institute of Biochemistry & Department of Biology, Carleton University;[2]Biochemistry Department, Schulich School of Medicine and Dentistry, Western University;[3]Department of Biology, Genetics Institute, University of Florida;[4]Department of Surgery & Center for Engineering in Medicine, Massachusetts General Hospital & Harvard Medical School;[5]Chemistry and Chemical Engineering Department, Royal Military College of Canada;[6]UMR 7179 Centre National de la Recherche Scientifique, Muse'um National d'Histoire Naturelle 出　　处：《Genomics, Proteomics & Bioinformatics》索引2015年第13卷第2期,共8页 基　　金：supported by a Discovery grant from the Natural Sciences and Engineering Research Council (NSERC) of Canada (Grant No. 6793);a grant from the Heart and Stroke Foundation of Canada (Grant No. G-140005874) to KBS. KBS holds the Canada Research Chair in Molecular Physiology;SNT, KKB, and CWW all held NSERC postgraduate scholarships 摘　　要：A variety of mammals employ torpor as an energy-saving strategy in environments of marginal or severe stress either on a daily basis during their inactive period or on a seasonal basis during prolonged multi-day hibernation. Recently, a few Madagascar lemur species have been identified as the only primates that exhibit torpor; one of these is the gray mouse lemur(Microcebus murinus). To explore the regulatory mechanisms that underlie daily torpor in a primate, we analyzed the expression of 28 selected genes that represent crucial survival pathways known to be involved in squirrel and bat hibernation. Array-based real-time PCR was used to compare gene expression in control(aroused) versus torpid lemurs in five tissues including the liver, kidney,skeletal muscle, heart, and brown adipose tissue. Significant differences in gene expression during torpor were revealed among genes involved in glycolysis, fatty acid metabolism, antioxidant defense, apoptosis, hypoxia signaling, and protein protection. The results showed upregulation of select genes primarily in liver and brown adipose tissue. For instance, both tissues showed elevated gene expression of peroxisome proliferator activated receptor gamma(ppargc), ferritin(fth1), and protein chaperones during torpor. Overall, the data show that the expression of only a few genes changed during lemur daily torpor, as compared with the broader expression changes reported for hibernation in ground squirrels. These results provide an indication that the alterations in gene expression required for torpor in lemurs are not as extensive as those needed for winter hibernation in squirrel models. However, identification of crucial genes with altered expression that support lemur torpor provides key targets to be explored and manipulated toward a goal of translational applications of inducible torpor as a treatment option in human biomedicine. 关 键 词：基因表达 狐猴 过氧化物酶体增殖物激活受体 天麻 灰色 褐色脂肪组织 灵长类动物 老鼠