Identification of the miniature pig inbred line by skin allograft

查看全文 作　　者：MU Yu-[1]lian;LIU [1]Lan;FENG Shu-[1]tang;WU Tian-[1]wen;LI [1]Kui;LI Jun-[2]you;HE [1]Wei;GAO [1]Qian;ZHOU Wen-[2]fang;WEI Jing-[1]liang;TANG [1]Fang;YANG Shu-[1]lin;WU Zhi-[2]gu;XIA [1]Ying;SUN Tong-[2]zhu

机构地区：[1]Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences;[2]Key Laboratory of Wound Repair, the General Hospital of the People's Liberation Army

出　　处：《Journal of Integrative Agriculture》索引2015年第14卷第7期,共7页

基　　金：supported by the National High Technology Research and Development Program of China (2012AA020603);the National Transgenic Major Project, China (2008ZX08012-002-05);the National Key Technoligy R&D Program of China (2012BA13904) 摘　　要：Skin grafting has been used as one of the most reliable tests to determine the genetic stability of laboratory animal such as mice and rats inbred line, but no identification of swine inbred lines by skin grafting has been reported. At present, Wuzhishan miniature pig(WZSP) inbred line has acquired the F24 individuals in China. In order to verify whether WZSP inbred line had been cultivated successfully, allogeneic skin grafts and related research were performed on F20 individuals of WZSP inbreeding population, compared with a control group of autologous transplantation. We observed the transplant recipients' wounds, detected peripheral blood-related indicators interleukin-2, 4 and 10, CD4+ and CD8+ lymphocytes, and conducted hematoxylin-eosin(HE) and Masson's staining of skin to judge whether the immune rejection reactions occurred within 28 days after transplantation. Chr. 7 genomic heterozygosity of 48 WZSP individuals from F20 to F22 was analyzed by high-density single nucleotide polymorphism(SNP) chips(60 000 SNPs). The result showed that there were no significant differences in graft skin, the plasma interleukin-2, 4, 10, CD4+ and CD8+, HE and Masson's staining results between the allograft and autograft groups, and no immune rejection occurred on the allograft group. We found that 11 genes in Chr. 7 of major histocompatibility complex(MHC) I and MHC II were homozygous which confirmed that immune antibody of the allograft and autograft groups were highly identical and also provided a theoretical basis to no immune rejection occurred on the allograft in the inbred WZSP. The result proved that the WZSP inbred line had been cultivated successfully for the first time in the world. The test methods also provide a scientific basis for the identification of swine and mammal inbred lines. 关键词：皮肤移植小型猪近交系鉴定主要组织相容性复合体白细胞介素-2 免疫排斥反应单核苷酸多态性

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Identification of the miniature pig inbred line by skin allograft

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