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Ultrasonic Extraction of Polysaccharides from Laminaria japonica and Their Antioxidative and Glycosidase Inhibitory Activities

查看全文 作　　者：WAN [1,2]Peng;YANG [3]Xiaoman;CAI [1]Bingna;CHEN [1]Hua;SUN [1]Huili;CHEN [1,2]Deke;PAN [1]Jianyu 高影响力作者 机构地区：[1]CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences;[2]University of Chinese Academy of Sciences;[3]Ocean University of China高影响力机构 出　　处：《Journal of Ocean University of China》索引2015年第14卷第4期,共12页高影响力期刊 基　　金：supported by the Project of National Key Technology Research and Development Program for the 12th Five-year Plan(No.2012BAD33B10);Public Science and Technology Research Funds Projects of Ocean(No.201305018-2);the Innovative Development of Marine Economy Regional Demonstration Projects(Nos.SZHY2012-B01-004,GD2013-B03-001);the National Scien-ce Foundation for Young Scientists of China(No.31101271);the Natural Science Foundation of Guangdong Province(Nos.2014A030310338,2014A030310351);the Comprehensive Strategic Cooperation Programs between the Guangdong Province and Chinese Academy of Sciences(No.2011B090300057);the Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology,Chinese Academy of Science(No.SQ 201017) 摘　　要：In the present study, ultrasonic extraction technique(UET) is used to improve the yield of polysaccharides from Laminaria japonica(LJPs). And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design(BBD) combined with response surface methodology(RSM) is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80℃ and ratio of material to solvent at 1:50(g m L-1). Under these optimal ultrasonic extraction conditions, an actual experimental yield(5.75% ± 0.3%) is close to the predicted result(5.67%) with no significant difference(P > 0.05). Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides(LJP) and two major ethanol precipitated fractions(LJP1 and LJP2) are in a concentration-dependent manner. LJP2(30%-60% ethanol precipitated polysaccharides) possesses the strongest α-glucosidase inhibitory activity and moderate scavenging activity against hydroxyl radicals(66.09% ± 2.19%, 3.0 mg m L-1). Also, the inhibitory activity against α-glucosidase(59.08% ± 3.79%, 5.0 mg m L-1) is close to that of acarbose(63.99% ± 3.27%, 5.0 mg m L-1). LJP1(30% ethanol precipitated polysaccharides) exhibits the strongest scavenging activity against hydroxyl radicals(99.80% ± 0.00%, 3.0 mgm L-1) and moderate α-glucosidase inhibitory activity(47.76% ± 1.92%, 5.0 mg m L-1). LJP shows the most remarkable DPPH scavenging activity(66.20% ± 0.11%, 5.0 mg m L-1) but weakest α-glucosidase inhibitory activity(37.77% ± 1.30%, 5.0 mg m L-1). However, all these LJPs exert weak inhibitory effects against α-amylase. These results show that UET is an effective method for extracting bioactive polysaccharides from seaweed materials. LJP1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of diabetes. This study provides scientific evidence and advances in the preparation technology and a hypoglycemic activities evaluation method for seaweed polysaccharides, especially glycosidase inhibition in combination with an antioxidative activity evaluation method. 关 键 词：生物活性多糖 超声波提取 抗氧化活性 海带多糖 BOX-BEHNKEN设计 Α-葡萄糖苷酶 羟基自由基 抑制活性
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